Infectious Diseases & Immunology Division, CSIR- Indian Institute of Chemical Biology, Kolkata 700032, India.
Regional Radiation Medicine Center, Thakurpukur Cancer Center & Welfare Home Campus, Kolkata 700063, India.
Nanomedicine (Lond). 2019 Aug;14(15):2045-2065. doi: 10.2217/nnm-2019-0022. Epub 2019 Aug 1.
Garcinol (GAR)-loaded cationic nanoliposomes were developed to achieve potential antitumor efficacy on B16F10 melanoma cells and . Two different phospholipids namely, distearoyl phosphatidylcholine (DSPC) and dipalmitoyl phosphatidylcholine (DPPC) were used in formulation to elucidate the difference in cellular uptake, cytotoxicity, tumor uptake (by scintigraphic imaging after technetium-99m radiolabeling) and therapeutic efficacy. Different protocols, for example, MTT assay, apoptosis study, gene expression analysis, chromatin condensation and cytoskeleton breakdown analysis in B16F10 cell lines as well as scintigraphic analysis and tumor inhibition studies (B16F10 tumor xenograft model) revealed superiority of GAR-DPPC than GAR-DSPC and free GAR in melanoma prevention. Cationic nanoliposomal formulations could be a future medication for skin cancer treatment.
姜酚（GAR）负载的阳离子纳米脂质体的开发旨在实现对 B16F10 黑色素瘤细胞的潜在抗肿瘤疗效。使用两种不同的磷脂，即二硬脂酰基磷脂酰胆碱（DSPC）和二棕榈酰基磷脂酰胆碱（DPPC）来阐明细胞摄取、细胞毒性、肿瘤摄取（通过锝-99m 放射性标记后的闪烁成像）和治疗效果的差异。不同的方案，例如 MTT 测定、凋亡研究、基因表达分析、B16F10 细胞系中的染色质浓缩和细胞骨架分解分析以及闪烁成像分析和肿瘤抑制研究（B16F10 肿瘤异种移植模型）表明 GAR-DPPC 比 GAR-DSPC 和游离 GAR 在预防黑色素瘤方面更具优势。阳离子纳米脂质体制剂可能成为未来治疗皮肤癌的药物。
