Laboratory of Aromatic and Medicinal Plants (LPAM), Center of Biotechnology, Borj Cedria, Tunisia.
INSERM UMR1231, Equipe Physiologie de la Nutrition & Toxicologie Université de Bourgogne -Franche-Comté, Dijon, France.
J Food Biochem. 2019 Aug;43(8):e12940. doi: 10.1111/jfbc.12940. Epub 2019 Jun 4.
Triterpenoids, tocopherols, and phytosterols presented in unsaponifiable fraction of grape seed oil have several beneficial effects comprising antioxidant, anti-inflammatory, and antitumor capacities. In this study, the unsaponifiable fraction of three Tunisian grape seed varieties (Vitis vinifera L.), namely Merlot, Carignan, and Syrah, was investigated. The identified compounds were two triterpenic compounds (β-amyrin, lanosterol), six phytosterols (campesterol, ∆ -avenasterol, stigmasterol, β-sitosterol, β-sitostanol, cholesterol), and three tocopherols (α, β, and γ tocopherols). The unsaponifiable fraction had significant protection against oxidative damage by modulating NO production and antioxidant activity. Statistical analysis showed the presence of three clusters of varieties associated to specific composition patterns. These results clearly demonstrated that unsaponifiable fraction profiles of grape species could be considered as a complementary data to the existing taxonomic evidence and classification purposes. PRACTICAL APPLICATIONS: Recently, much attention has been focused to substitute artificial antioxidant by others originating from natural products as plant matrices. The unsaponifiable fraction of grape seed oils is an interesting source of bioactive components like phytosterols, tocopherols, triterpenoids, and other various components. These components are known for their antitumor, anti-inflammatory and antioxidant capacities.
葡萄籽油不皂化物部分中存在的三萜类化合物、生育酚和植物甾醇具有多种有益作用，包括抗氧化、抗炎和抗肿瘤能力。在这项研究中，研究了三种突尼斯葡萄籽油品种（Vitis vinifera L.），即梅洛、佳利酿和西拉的不皂化物部分。鉴定出的化合物有两种三萜类化合物（β-香树脂醇、羊毛甾醇）、六种植物甾醇（菜油甾醇、Δ-avenasterol、豆甾醇、β-谷甾醇、β-谷甾烷醇、胆固醇）和三种生育酚（α、β 和 γ 生育酚）。不皂化物部分通过调节 NO 产生和抗氧化活性，对氧化损伤具有显著的保护作用。统计分析显示，存在三个与特定组成模式相关的品种群。这些结果清楚地表明，葡萄品种的不皂化物部分图谱可以被认为是对现有分类证据和分类目的的补充数据。实际应用：最近，人们越来越关注用其他天然产品，如植物基质，替代人工抗氧化剂。葡萄籽油的不皂化物部分是生物活性成分（如植物甾醇、生育酚、三萜类化合物和其他各种成分）的一个有趣来源。这些成分因其抗肿瘤、抗炎和抗氧化能力而闻名。
