Center of Excellence in Biotechnology Research, College of Science, King Saud University, Riyadh, Saudi Arabia.
Research Chair for Biomedical Applications of Nanomaterials, Biochemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
J Food Biochem. 2019 Aug;43(8):e12962. doi: 10.1111/jfbc.12962. Epub 2019 Jun 28.
In the present study, we tested the antioxidant and anti-inflammatory potential of the plant flavonoid, fisetin against cigarette smoke-induced oxidative stress, and inflammation in rat lungs. Male Wistar rats were chronically exposed to cigarette smoke (CS) with or without administration of fisetin. Fisetin administration to CS-exposed rats resulted in a significant reduction in neutrophils and macrophages in bronchoalveolar lavage fluid as well as malondialdehyde, 3-nitrotyrosine, 8-isoprostane, tumor necrosis factor-alpha, interleukin-1beta, granulocyte macrophage-colony stimulating factor, interleukin-4, and interleukin-10 levels in lung tissues compared to those in CS-exposed rats not treated with fisetin. Fisetin also significantly augmented lung hemoxinase-1, glutathione peroxidase-2, reduced glutathione, superoxide dismutase, nitric oxide, and nuclear factor erythroid 2-related factor (Nrf2) levels in CS-exposed rats. In addition, a marked reversal in CS-induced histopathological changes was noted in fisetin-treated rats. Collectively, these data demonstrate the potential of fisetin to blunt CS-induced oxidative stress and inflammation in the lung and to prevent tissue damage via the Nrf2-mediated upregulation of antioxidant gene expression. PRACTICAL APPLICATIONS: In the present study, we found that the plant flavonoid, fisetin significantly abrogated the oxidative stress, inflammation, and tissue damage induced by cigarette smoke, a powerful pro-oxidant in rat lungs. Additionally, fisetin markedly reversed cigarette smoke-induced increases in neutrophil and macrophage cell populations in bronchoalveolar lavage fluid. These findings are particularly significant considering the association of cigarette smoking with increased oxidative stress and inflammation, which are central to the pathologies of a wide variety of chronic diseases including chronic obstructive pulmonary disease, cancer, and cardiovascular diseases. Therefore, the present work underscores the beneficial effects of the regular consumption of plant-based foods with medicinal properties for the effective prevention of these chronic diseases.
在本研究中，我们测试了植物类黄酮漆黄素对香烟烟雾引起的大鼠肺部氧化应激和炎症的抗氧化和抗炎潜力。雄性 Wistar 大鼠被长期暴露于香烟烟雾中，同时或不给予漆黄素治疗。与未用漆黄素治疗的 CS 暴露大鼠相比，漆黄素给药可使支气管肺泡灌洗液中的中性粒细胞和巨噬细胞以及肺组织中的丙二醛、3-硝基酪氨酸、8-异前列腺素、肿瘤坏死因子-α、白细胞介素-1β、粒细胞巨噬细胞集落刺激因子、白细胞介素-4 和白细胞介素-10 水平显著降低。漆黄素还显著增加了 CS 暴露大鼠的血红素加氧酶-1、谷胱甘肽过氧化物酶-2、还原型谷胱甘肽、超氧化物歧化酶、一氧化氮和核因子红细胞 2 相关因子（Nrf2）水平。此外，在漆黄素治疗的大鼠中观察到 CS 诱导的组织病理学变化明显逆转。总之，这些数据表明，漆黄素具有减弱 CS 引起的肺部氧化应激和炎症的潜力，并通过 Nrf2 介导的抗氧化基因表达上调来防止组织损伤。实际应用：在本研究中，我们发现植物类黄酮漆黄素可显著减轻香烟烟雾引起的氧化应激、炎症和组织损伤，香烟烟雾是大鼠肺部的一种强氧化剂。此外，漆黄素显著逆转了香烟烟雾诱导的支气管肺泡灌洗液中中性粒细胞和巨噬细胞数量的增加。考虑到吸烟与氧化应激和炎症的增加有关，这些发现尤为重要，氧化应激和炎症是多种慢性疾病（包括慢性阻塞性肺疾病、癌症和心血管疾病）病理的核心。因此，本工作强调了经常食用具有药用特性的植物性食物对有效预防这些慢性疾病的有益作用。
