The Laboratory of Cancer Biology and Cannabinoid Research, Department of Biology , Technion-Israel Institute of Technology , Haifa 320003 , Israel.
Anal Chem. 2019 Sep 3;91(17):11425-11432. doi: 10.1021/acs.analchem.9b02844. Epub 2019 Aug 15.
The therapeutic effect of largely depends on the content of its pharmacologically active secondary metabolites, mainly phytocannabinoids, flavonoids, and terpenoids. Recent studies suggest there are therapeutic effects of specific terpenoids as well as synergistic effects with other active compounds in the plant. Although contains an overwhelming milieu of terpenoids, only a limited number are currently reported and used for metabolic analysis of chemovars. In this study, we report the development and validation of a method for simultaneous quantification of 93 terpenoids in air-dried inflorescences and extracts. This method employs the full evaporation technique via a static headspace sampler, followed by gas chromatography-mass spectrometry (SHS-GC/MS/MS). In the validation process, spiked terpenoids were quantified with acceptable repeatability, reproducibility, sensitivity, and accuracy. Three medical chemovars were used to study the effect of sample preparation and extraction methods on terpenoid profiles. This method was further applied for studying the terpenoid profiles of 16 different chemovars acquired at different dates. Our results demonstrate that sample preparation methods may significantly impact the chemical fingerprint compared to the nontreated . This emphasizes the importance of performing SHS extraction in order to study the natural terpenoid contents of chemovars. We also concluded that most inflorescences expressed relatively unique terpenoid profiles for the most pronounced terpenoids, even when sampled at different dates, although absolute concentrations may vary due to aging. The suggested method offers an ideal tool for terpenoid profiling of and sets the scene for more comprehensive works in the future.
的治疗效果在很大程度上取决于其具有药理活性的次生代谢产物的含量，主要是植物大麻素、类黄酮和萜类化合物。最近的研究表明，特定萜类化合物以及它们与植物中其他活性化合物的协同作用具有治疗效果。尽管含有压倒性的萜类混合物，但目前只有有限数量的萜类化合物被报道并用于代谢分析。在这项研究中，我们报告了一种同时定量分析风干花序和提取物中 93 种萜类化合物的方法的开发和验证。该方法采用静态顶空采样器通过全蒸发技术，随后进行气相色谱-质谱（SHS-GC/MS/MS）分析。在验证过程中，用可接受的重复性、重现性、灵敏度和准确度对加标萜类化合物进行了定量。使用三种医用大麻品种研究了样品制备和提取方法对萜类化合物图谱的影响。该方法进一步应用于研究在不同日期采集的 16 个不同品种的萜类化合物图谱。我们的结果表明，与未处理的相比，样品制备方法可能会对化学指纹图谱产生显著影响。这强调了进行 SHS 提取以研究品种的天然萜类化合物含量的重要性。我们还得出结论，即使在不同日期采样，大多数花序也表现出相对独特的萜类化合物图谱，对于最明显的萜类化合物来说，尽管由于老化，绝对浓度可能会有所不同。该方法为研究提供了理想的工具，并为未来更全面的工作奠定了基础。
