Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Palatucci, Polo Universitario Annunziata, 98168 Messina, Italy.
Department of Mathematical and Computer Science, Physical Sciences and Earth Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres 31, 98166 Messina, Italy.
Molecules. 2021 Mar 13;26(6):1588. doi: 10.3390/molecules26061588.
A microwave distillation method was optimized for the extraction and isolation of cannabis essential oil from fresh and dried hemp inflorescences. The developed method enabled us to obtain a distilled product rich in terpenes and terpenoid compounds, responsible of the typical and unique smell of the cannabis plant. The distillate from different hemp cultivars, including Kompolti, Futura 75, Carmagnola, Felina 32 and Finola were characterized by using a gas chromatograph equipped with both mass spectrometer and flame ionization detectors. In a single chromatographic run, the identity and absolute amounts of distilled compounds were determined. Peak assignment was established using a reliable approach based on the usage of two identification parameters, named reverse match, and linear retention index filter. Absolute quantification (mg g) of the analytes was performed using an internal standard method applying the flame ionization detector (FID) response factors according to each chemical family. An enantio-GC-MS method was also developed in order to evaluate the enantiomeric distribution of chiral compounds, an analytical approach commonly utilized for establishing the authenticity of suspicious samples.
一种微波蒸馏方法被优化用于从新鲜和干燥的大麻花序中提取和分离大麻精油。所开发的方法使我们能够获得一种富含萜烯和萜类化合物的蒸馏产物，这些化合物是大麻植物典型和独特气味的原因。使用配备质谱仪和火焰离子化检测器的气相色谱仪对来自不同大麻品种的馏出物进行了表征，包括 Kompolti、Futura 75、Carmagnola、Felina 32 和 Finola。在单次色谱运行中，确定了蒸馏化合物的身份和绝对含量。通过使用基于使用两种鉴定参数的可靠方法，即反向匹配和线性保留指数过滤器，建立了峰分配。使用内部标准法，根据每个化学家族应用火焰离子化检测器 (FID) 的响应因子，对分析物进行绝对定量（mg g）。还开发了一种对映体 GC-MS 方法，以评估手性化合物的对映体分布，这是一种常用于确定可疑样品真实性的分析方法。
