Laboratory of Forest Pathogen Integrated Biology, Research Institute of Forestry New Technology, Chinese Academy of Forestry, Beijing 100091, China.
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Phytopathology. 2019 Dec;109(12):2009-2021. doi: 10.1094/PHYTO-12-18-0452-R. Epub 2019 Nov 4.
Poplar are important forestry species in China, but the pathogen causes serious economic losses worldwide. To identify candidate resistance proteins and explore the molecular mechanisms involved in poplar-pathogen interactions, proteomic responses of stem samples from resistant and susceptible poplar ecotypes to were investigated using nanoflow liquid chromatography-tandem mass spectrometry with label-free quantitative analysis. We identified 588 proteins, divided into 21 biological process categories including 48 oxidoreductases, 72 hydrolytic enzymes, 80 metabolic enzymes, and 29 proteins of unknown function. Differential proteome analysis revealed large differences between resistant Carr and susceptible Hsu ecotypes before and after inoculation. Among 102 identified proteins, 22 were highly upregulated in the resistant genotype but downregulated in the susceptible genotype. Proteins induced in Carr in response to are associated with plant defenses including oxidoreductase activity (catalase, isocitrate dehydrogenase, and superoxide dismutase), phenylpropanoid biosynthesis and phenylalanine metabolism (alcohol dehydrogenase), photosynthesis (ATP synthase subunit alpha, ATP synthase gamma chain, photosystem I P700 chlorophyll a apoprotein A2, photosystem II CP47 chlorophyll apoprotein), carbon fixation (pyruvate kinase, triosephosphate isomerase, malic enzyme, phosphoglycerate kinase, ribulose-1,5-bisphosphate carboxylase, and ribulose bisphosphate carboxylase small chain), and glycolysis/gluconeogenesis (fructose-bisphosphate aldolase). Kyoto Encyclopedia of Genes and Genomes pathway analysis identified 168 proteins related to metabolic pathways, 41 proteins related to the biosynthesis of phenylpropanoids, and 36 proteins related to the biosynthesis of plant hormones, the biosynthesis of alkaloids derived from ornithine, lysine, and nicotinic acid, and photosynthesis in response to . Our findings provide insight into plant-pathogen interactions in resistant and susceptible poplar ecotypes infected with and could assist the development of novel strategies for fighting poplar canker disease.
杨属是中国重要的林业树种，但该病原体在全球范围内造成了严重的经济损失。为了鉴定杨树抗源中潜在的抗病蛋白并探讨杨树与病原菌互作的分子机制，本研究采用无标记定量分析的纳升液相色谱-串联质谱技术，对来自抗病和感病杨树生态型的茎样进行了蛋白质组学研究。共鉴定到 588 种蛋白质，分为 21 个生物学过程，包括 48 种氧化还原酶、72 种水解酶、80 种代谢酶和 29 种未知功能蛋白。差异蛋白质组学分析显示，接种前后抗病品种 Carr 和感病品种 Hsu 之间存在很大差异。在鉴定到的 102 种蛋白中，有 22 种在抗病基因型中高度上调，而在感病基因型中下调。Carr 对 诱导的蛋白与植物防御相关，包括氧化还原酶活性（过氧化氢酶、异柠檬酸脱氢酶和超氧化物歧化酶）、苯丙烷生物合成和苯丙氨酸代谢（醇脱氢酶）、光合作用（ATP 合酶亚基α、ATP 合酶γ链、PSI P700 叶绿素 a 脱辅基蛋白 A2、PSII CP47 叶绿素 a 脱辅基蛋白）、碳固定（丙酮酸激酶、磷酸丙糖异构酶、苹果酸酶、磷酸甘油酸激酶、核酮糖-1,5-二磷酸羧化酶、核酮糖-1,5-二磷酸羧化酶小链）和糖酵解/糖异生（果糖二磷酸醛缩酶）。京都基因与基因组百科全书通路分析鉴定出与代谢途径相关的 168 种蛋白、与苯丙烷生物合成相关的 41 种蛋白和与植物激素生物合成相关的 36 种蛋白，以及与 诱导的类黄酮生物合成相关的 36 种蛋白。本研究结果为了解抗病和感病杨树生态型感染 后的植物-病原菌互作提供了新的见解，并为防治杨树溃疡病提供了新的策略。
