Wang Yue-Feng, Hou Xue-Yue, Deng Jun-Jie, Yao Zhi-Hong, Lyu Man-Man, Zhang Rong-Shu
College of Landscape Architecture, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Photosynthesis Research Center, CAS Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Plants (Basel). 2020 Feb 19;9(2):272. doi: 10.3390/plants9020272.
Numerous strains have been reported to be optimal biofertilizers and biocontrol agents with low production costs and environmentally friendly properties. spp. promote the growth and immunity of plants by multiple means. Interfering with the hormonal homeostasis in plants is the most critical strategy. However, the mechanisms underlying plants' responses to remain to be further elucidated. Auxin is the most important phytohormone that regulates almost every aspect of a plant's life, especially the trade-off between growth and defense. The AUXIN RESPONSE FACTOR (ARF) family proteins are key players in auxin signaling. We studied the responses and functions of the gene in a hybrid poplar during its interaction with beneficial . strains using transformed poplar plants with overexpression (on transcription level in this study). We report that is a positive regulator for promoting poplar growth and defense responses, as does . inoculation. also turned out to be a positive stimulator of adventitious root formation. Particularly, the overexpression of induced a 32.3% increase in the height of 40-day-old poplar plants and a 258% increase in the amount of adventitious root of 3-week-old subcultured plant clones. Overexpressed exerted its beneficial functions through modulating the hormone levels of indole acetic acid (IAA), jasmonic acid (JA), and salicylic acid (SA) in plants and activating their signaling pathways, creating similar results as inoculated with . Particularly, in the overexpressing poplar plants, the IAA level increased by approximately twice of the wild-type plants; and the signaling pathways of IAA, JA, and SA were drastically activated than the wild-type plants under pathogen attacks. Our report presents the potential of s as the crucial and positive responders in plants to inducing.
据报道，许多菌株是理想的生物肥料和生物防治剂，生产成本低且具有环境友好特性。[具体菌种名称]通过多种方式促进植物生长和免疫。干扰植物体内的激素稳态是最关键的策略。然而，植物对[具体菌种名称]反应的潜在机制仍有待进一步阐明。生长素是最重要的植物激素，几乎调节植物生命的各个方面，尤其是生长与防御之间的权衡。生长素响应因子（ARF）家族蛋白是生长素信号传导的关键参与者。我们研究了杂交杨树中[具体基因名称]基因在与有益[具体菌种名称]菌株相互作用过程中的反应和功能。我们使用[具体基因名称]过表达的转基因杨树植株（本研究中在转录水平上）进行研究。我们报告称，[具体基因名称]是促进杨树生长和防御反应的正调控因子，就像接种[具体菌种名称]菌株一样。[具体基因名称]接种也被证明是不定根形成的正刺激因子。特别是，[具体基因名称]的过表达使40日龄杨树植株高度增加了32.3%，3周龄继代培养植物克隆的不定根数量增加了258%。过表达的[具体基因名称]通过调节植物中吲哚乙酸（IAA）、茉莉酸（JA）和水杨酸（SA）的激素水平并激活它们的信号通路发挥其有益功能，产生与接种[具体菌种名称]菌株相似的结果。特别是，在过表达的杨树植株中，IAA水平比野生型植株增加了约两倍；在病原体攻击下，IAA、JA和SA的信号通路比野生型植株被大幅激活。我们的报告揭示了[具体菌种名称]作为植物中对[具体菌种名称]诱导的关键和积极响应者的潜力。
