Department of Medical Physiology, School of Medicine, University of Nairobi, GPO 30197-00100, Kenya.
J Ethnopharmacol. 2019 Nov 15;244:112077. doi: 10.1016/j.jep.2019.112077. Epub 2019 Jul 29.
Rotheca myricoides (Hochst.) Steane & Mabb is a plant species used in traditional medicine for the management of diabetes in the lower eastern part of Kenya (Kitui, Machakos and Makueni Counties, Kenya) that is mainly inhabited by the Kamba community.
This study investigated the antihyperglycaemic, antidyslipidemic and antihyperinsulinemic activity of the freeze-dried extracts of Rotheca myricoides (Hochst.) Steane & Mabb (RME) in an animal model of type 2 diabetes mellitus.
Type 2 diabetes was induced by dietary manipulation for 56 days via (high fat- high fructose diet) and intraperitoneal administration of streptozocin (30 mg/kg). Forty freshly-weaned Sprague Dawley rats were randomly assigned into the negative control (high fat/high fructose diet), low dose test (50mg/kg RME, high dose test (100mg/kg RME and positive control (Pioglitazone, 20mg/kg) groups. Fasting blood glucose and body weight were measured at weekly intervals. Oral glucose tolerance tests were performed on days 28 and 56. Lipid profile, hepatic triglycerides, fasting serum insulin levels and serum uric acid were determined on day 56.
The RME possessed significant antihyperglycemic [FBG: 6.5 ± 0.11 mmol/l (negative control) vs. 4.62 ± 0.13 mmol/l (low dose test) vs. 5.25 ± 0.15 mmol/l in (high dose test) vs. 4.33 ± 0.09 mmol/l (positive control): p < 0.0001] and antihyperinsulinemic effects [1.84 ± 0.19 (negative control) vs. (0.69 ± 0.13 (low dose test) vs. (0.83 ± 0.17 (high dose test) vs. (0.69 ± 0.10 (positive control): F (3, 36) = 0.6421: p < 0.0001. The extracts also possessed significant antidyslipidemic effects [LDL levels: 3.52 ± 0.19 mmol/l (negative control) vs. 0.33 ± 0.14 mmol/l (low dose test) vs. 0.34 ± 0.20 mmol/l (high dose test) vs. 0.33 ± 0.01 mmol/l (positive control): p < 0.0001].RME significantly lowered plasma uric acid levels, as well as hepatic triglycerides and hepatic weights. Network pharmacology analysis indicated that the observed pharmacological effects are mediated via the modulation of Peroxisome proliferator-activated gamma receptor.
The freeze dried extracts of Rotheca myricoides possessed significant antihyperglycemic and antidyslidemic effects. In addition it lowered serum uric levels, as well as hepatic triglycerides and hepatic weight. These results appear to validate the traditional use of this plant species in the management of diabetes mellitus.
Rotheca myricoides（Hochst.）Steane & Mabb 是一种植物物种，在肯尼亚下东部（肯尼亚基图伊、马查科斯和马库埃尼县）的传统医学中用于治疗糖尿病，主要由康巴社区居住。
本研究旨在研究 Rotheca myricoides（Hochst.）Steane & Mabb（RME）冻干提取物在 2 型糖尿病动物模型中的抗高血糖、抗脂血症和抗高胰岛素血症活性。
通过（高脂肪-高果糖饮食）和腹腔内给予链脲佐菌素（30mg/kg），通过饮食操作诱导 2 型糖尿病 56 天。40 只刚断奶的 Sprague Dawley 大鼠随机分为阴性对照组（高脂肪/高果糖饮食）、低剂量测试组（50mg/kg RME、高剂量测试组（100mg/kg RME）和阳性对照组（吡格列酮，20mg/kg）。每周测量一次空腹血糖和体重。在第 28 天和第 56 天进行口服葡萄糖耐量试验。第 56 天测定血脂谱、肝甘油三酯、空腹血清胰岛素水平和血清尿酸。
RME 具有显著的抗高血糖作用[FBG：6.5±0.11mmol/l（阴性对照）vs.4.62±0.13mmol/l（低剂量测试）vs.5.25±0.15mmol/l（高剂量测试）vs.4.33±0.09mmol/l（阳性对照）：p<0.0001]和抗高胰岛素血症作用[1.84±0.19（阴性对照）vs.（0.69±0.13（低剂量测试）vs.（0.83±0.17（高剂量测试）vs.（0.69±0.10（阳性对照）：F（3,36）=0.6421：p<0.0001。提取物还具有显著的抗脂血症作用[LDL 水平：3.52±0.19mmol/l（阴性对照）vs.0.33±0.14mmol/l（低剂量测试）vs.0.34±0.20mmol/l（高剂量测试）vs.0.33±0.01mmol/l（阳性对照）：p<0.0001]。RME 可显著降低血浆尿酸水平以及肝甘油三酯和肝重。网络药理学分析表明，观察到的药理作用是通过过氧化物酶体增殖物激活受体的调节介导的。
Rotheca myricoides 的冻干提取物具有显著的抗高血糖和抗脂血症作用。此外，它还降低了血清尿酸水平以及肝甘油三酯和肝重。这些结果似乎验证了该植物物种在糖尿病治疗中的传统用途。
