Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, 14 Prusa St., 08-110 Siedlce, Poland.
Department of Molecular Biotechnology, University of Rzeszow, 1 Pigonia St., 35-310 Rzeszow, Poland.
Int J Mol Sci. 2019 Jul 31;20(15):3742. doi: 10.3390/ijms20153742.
Prior experiments illustrated reactive oxygen species (ROS) overproduction in maize plants infested with bird-cherry-oat ( L.) aphids. However, there is no available data unveiling the impact of aphids feeding on oxidative damages of crucial macromolecules in maize tissues. Therefore, the purpose of the current study was to evaluate the scale of oxidative damages of genomic DNA, total RNA and mRNA, proteins, and lipids in seedling leaves of two maize genotypes (Złota Karłowa and Waza cvs-susceptible and relatively resistant to the aphids, respectively). The content of oxidized guanosine residues (8-hydroxy-2'-deoxyguanosine; 8-OHdG) in genomic DNA, 8-hydroxyguanosine (8-OHG) in RNA molecules, protein carbonyl groups, total thiols (T-SH), protein-bound thiols (PB-SH), non-protein thiols (NP-SH), malondialdehyde (MDA) and electrolyte leakage (EL) levels in maze plants were determined. In addition, the electrical penetration graphs (EPG) technique was used to monitor and the aphid stylet positioning and feeding modes in the hosts. Maize seedlings were infested with 0 (control), 30 or 60 adult apterae per plant. Substantial increases in the levels of RNA, protein and lipid oxidation markers in response to aphid herbivory, but no significant oxidative damages of genomic DNA, were found. Alterations in the studied parameters were dependent on maize genotype, insect abundance and infestation time.
先前的实验表明，被鸟-樱桃-燕麦（L.）蚜虫侵害的玉米植株中活性氧（ROS）的过度产生。然而，目前还没有数据揭示蚜虫取食对玉米组织中关键大分子氧化损伤的影响。因此，本研究的目的是评估两种玉米基因型（Złota Karłowa 和 Waza，分别对蚜虫敏感和相对抗性）幼苗叶片中基因组 DNA、总 RNA 和 mRNA、蛋白质和脂质的氧化损伤程度。氧化鸟嘌呤残基（8-羟基-2'-脱氧鸟苷；8-OHdG）在基因组 DNA 中的含量、RNA 分子中的 8-羟基鸟嘌呤（8-OHG）、蛋白质羰基、总巯基（T-SH）、蛋白质结合巯基（PB-SH）、非蛋白质巯基（NP-SH）、丙二醛（MDA）和电解质渗漏（EL）水平在玉米植株中进行了测定。此外，还使用电穿透图谱（EPG）技术监测和记录蚜虫口器在宿主中的定位和取食模式。玉米幼苗被 0（对照）、30 或 60 只无翅若虫/株感染。蚜虫取食后，RNA、蛋白质和脂质氧化标记物的水平显著增加，但基因组 DNA 没有明显的氧化损伤。研究参数的变化取决于玉米基因型、昆虫丰度和感染时间。
