Calvo C, Ponsin G, Berthezene F
INSERM U 197, Laboratoire de Métabolisme des Lipides, Hôpital de l'Antiquaille, F-Lyon.
Diabete Metab. 1988 May-Jun;14(3):264-9.
In diabetic patients, hyperglycaemia results in the non enzymatic glycation of many proteins. We studied the glycation of HDL of patients with either type 1 or type 2 diabetes compared with that of control subjects. Although a basal glycation was detectable in HDL of normal individuals, this increased by about 400% in HDL of both groups of diabetic patients. The degree of HDL glycation was positively correlated with blood glucose concentration. All the HDL apoproteins were glycated but the glycation of apo A-I represented about 80% of the total HDL. These data were compared to those obtained in vitro after incubation of normal apo A-I either as free molecular species or as phospholipid/apo A-I complex, in the presence of glucose (0 to 80 mmol/l) at 37 degrees C. The resulting apo A-I glycation was dependent upon both time of incubation and glucose concentration and was largely increased in the presence of phospholipids. These data suggest that the in vivo glycation of HDL apoproteins might depend upon glucose concentration but might also be partly influenced by their lipid environment.
在糖尿病患者中，高血糖会导致许多蛋白质发生非酶糖基化。我们研究了1型或2型糖尿病患者高密度脂蛋白（HDL）的糖基化情况，并与对照组受试者进行了比较。虽然在正常个体的HDL中可检测到基础糖基化，但两组糖尿病患者HDL中的这种糖基化增加了约400%。HDL糖基化程度与血糖浓度呈正相关。所有HDL载脂蛋白都发生了糖基化，但载脂蛋白A-I（apo A-I）的糖基化约占HDL总糖基化的80%。将这些数据与在37℃下，正常apo A-I作为游离分子形式或磷脂/apo A-I复合物，在葡萄糖（0至80 mmol/L）存在下孵育后体外获得的数据进行比较。由此产生的apo A-I糖基化取决于孵育时间和葡萄糖浓度，并且在磷脂存在下大幅增加。这些数据表明，HDL载脂蛋白的体内糖基化可能取决于葡萄糖浓度，但也可能部分受其脂质环境的影响。
