Popoff M R, Rubin E J, Gill D M, Boquet P
Unité des Antigènes Bactériens, UA Centre National de la Recherche Scientifique, Paris, France.
Infect Immun. 1988 Sep;56(9):2299-306. doi: 10.1128/iai.56.9.2299-2306.1988.
By screening possible ADP-ribosyltransferase activities in culture supernatants from various Clostridium species, we have found one Clostridium difficile strain (CD196) (isolated in our laboratory) that is able to produce, in addition to toxins A and B, a new ADP-ribosyltransferase that was shown to covalently modify cell actin as Clostridium botulinum C2 or Clostridium perfringens E iota toxins do. The molecular weight of the CD196 ADP-ribosyltransferase (CDT) was determined to be 43 kilodaltons, and its isoelectric point was 7.8. No cytotoxic activity on Vero cells or lethal activity upon injection in mice was associated with this enzyme. CDT was neither related to C. difficile A or B toxins nor to C. botulinum C2 toxin component I. However, Vero cells cultivated in the presence of C. difficile B toxin had a lower amount of actin able to be ADP-ribosylated by CDT or C2 toxin in vitro. Antibodies raised against CDT reacted by immunoblot analysis with a 43-kilodalton protein of C. perfringens type E culture supernatant producing the iota toxin.
通过筛选各种梭菌属细菌培养上清液中可能存在的ADP-核糖基转移酶活性，我们发现了一株艰难梭菌菌株（CD196）（在我们实验室分离得到），该菌株除了能产生毒素A和毒素B外，还能产生一种新的ADP-核糖基转移酶，这种酶被证明能像肉毒梭菌C2毒素或产气荚膜梭菌E型iota毒素一样共价修饰细胞肌动蛋白。CD196 ADP-核糖基转移酶（CDT）的分子量测定为43千道尔顿，其等电点为7.8。该酶对Vero细胞无细胞毒性活性，注射到小鼠体内也无致死活性。CDT既与艰难梭菌A或B毒素无关，也与肉毒梭菌C2毒素成分I无关。然而，在艰难梭菌B毒素存在的情况下培养的Vero细胞，其体外能被CDT或C2毒素进行ADP-核糖基化的肌动蛋白量较低。针对CDT产生的抗体通过免疫印迹分析与产生iota毒素的产气荚膜梭菌E型培养上清液中的一种43千道尔顿蛋白发生反应。
