Diao Jun, Xie Jun, Feng Jianqi, Peng Cheng, Qi Chunmei, Li Li, Su Zhongping, Wu Weiheng
Chest Pain Center, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou.
Cardiology Department, Ganyu District People's Hospital of Lianyungang City.
Acta Cardiol Sin. 2019 Jul;35(4):355-359. doi: 10.6515/ACS.201907_35(4).20180922A.
This study measured the change in connective tissue growth factor levels after the onset of unstable angina and ST-segment elevation myocardial infarction, and studied its correlation with peak creatine kinase-MB (CK-MB) enzyme. It also discussed the significance of myocardial fibrosis after myocardial infarction. To detect the serum levels of connective tissue growth factor in patients with ST-segment elevation myocardial infarction and its relationship with the maximum level of CK-MB.
We selected 50 patients with ST-segment elevation myocardial infarction and 50 patients with unstable angina. Connective tissue growth factor levels were examined 24 h, 2 d, 7 d, and 14 d after the onset of ST-segment elevation myocardial infarction, and within 24 h for unstable angina, using enzyme-linked immunosorbent assay (ELISA). The maximum level of CK-MB was detected by immunosuppression.
The serum level of connective tissue growth factor in the unstable angina patients was 10.34 ± 2.00 ng/mL, and the levels in the ST-segment elevation myocardial infarction patients were 16.76 ± 3.17 ng/mL at 24 h, 29.87 ± 4.90 ng/mL at 2 d, 45.02 ± 8.35 ng/mL at 7 d, and 31.61 ± 4.40 ng/mL at 14 d. Compared with the unstable angina patients, the connective tissue growth factor levels in the ST-segment elevation myocardial infarction patients were significantly higher since day 1 (p < 0.01). The maximum level of CK-MB was correlated with connective tissue growth factor levels at 7 d (r = 0.859, p = 0.000).
Connective tissue growth factor was significantly expressed in the ST-segment elevation myocardial infarction patients, indicating that it might play an important role in myocardial fibrosis.
本研究测定了不稳定型心绞痛和ST段抬高型心肌梗死后结缔组织生长因子水平的变化，并研究其与肌酸激酶同工酶（CK-MB）峰值的相关性。还探讨了心肌梗死后心肌纤维化的意义。以检测ST段抬高型心肌梗死患者血清结缔组织生长因子水平及其与CK-MB最高水平的关系。
选取50例ST段抬高型心肌梗死患者和50例不稳定型心绞痛患者。采用酶联免疫吸附测定（ELISA）法，于ST段抬高型心肌梗死发病后24小时、2天、7天和14天检测结缔组织生长因子水平，不稳定型心绞痛患者在24小时内检测。采用免疫抑制法检测CK-MB的最高水平。
不稳定型心绞痛患者血清结缔组织生长因子水平为10.34±2.00 ng/mL，ST段抬高型心肌梗死患者在发病24小时时为16.76±3.17 ng/mL，2天时为29.87±4.90 ng/mL，7天时为45.02±8.35 ng/mL，14天时为31.61±4.40 ng/mL。与不稳定型心绞痛患者相比，ST段抬高型心肌梗死患者自第1天起结缔组织生长因子水平显著升高（p<0.01）。CK-MB的最高水平与7天时的结缔组织生长因子水平相关（r=0.859，p=0.000）。
结缔组织生长因子在ST段抬高型心肌梗死患者中显著表达，表明其可能在心肌纤维化中起重要作用。
