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苹果酸酶1（ME1）是胃癌细胞中的一种潜在癌基因，与胃癌患者的不良生存预后相关。

Malic enzyme 1 (ME1) is a potential oncogene in gastric cancer cells and is associated with poor survival of gastric cancer patients.

作者信息

Shi Yanyan, Zhou Siliang, Wang Pan, Guo Yanlei, Xie Bingteng, Ding Shigang

机构信息

Peking University Third Hospital, Research Center of Clinical Epidemiology, Beijing 100191, People's Republic of China.

Peking University Health Science Center, School of Public Health, Beijing 100191, People's Republic of China.

出版信息

Onco Targets Ther. 2019 Jul 11;12:5589-5599. doi: 10.2147/OTT.S203228. eCollection 2019.

DOI:10.2147/OTT.S203228
PMID:31371996
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6628973/
Abstract

BACKGROUND AND OBJECTIVE

Gastric cancer is one of the most common cancers worldwide. However, the mechanisms associated with this disease are still not clear. Malic enzyme 1 (ME1) is a metabolic enzyme that is overexpressed in various cancers. Here, we examined whether it is involved in gastric cancer.

METHODS

ME1 expression was knocked down in the gastric cancer cell line SGC7901. Cell growth and migration were measured using a real-time microelectronic cell sensor system. Cell invasion was measured using a Transwell assay. Cell cycle analysis was also performed to examine cell cycle arrest. A gastric cancer tissue microarray of gastric cancer was stained using immunohistochemistry. ME1 expression levels were also statistically analysed.

RESULTS

ME1 knockdown in gastric cancer SGC7901 cells significantly inhibited cell proliferation, migration, and invasion. Cell cycle arrest was induced in the G2 phase. Further, ME1 expression was significantly correlated with gastric cancer patient prognosis based on both univariable and multivariable survival analysis. No significant difference was found between ME1 expression in gastric cancer tissues and that in adjacent tissues.

CONCLUSION

Our results provide evidence that ME1 is a key factor for gastric cancer. ME1 might be pro-oncogenic during both the development and migration of gastric cancer; it also might be related to gastric cancer patient survival.

摘要

背景与目的

胃癌是全球最常见的癌症之一。然而，与这种疾病相关的机制仍不清楚。苹果酸酶1（ME1）是一种在多种癌症中过表达的代谢酶。在此，我们研究了它是否参与胃癌。

方法

在胃癌细胞系SGC7901中敲低ME1表达。使用实时微电子细胞传感器系统测量细胞生长和迁移。使用Transwell实验测量细胞侵袭。还进行细胞周期分析以检测细胞周期阻滞。用免疫组织化学对胃癌组织芯片进行染色。对ME1表达水平也进行了统计学分析。

结果

胃癌SGC7901细胞中ME1敲低显著抑制细胞增殖、迁移和侵袭。细胞周期在G2期被阻滞。此外，基于单变量和多变量生存分析，ME1表达与胃癌患者预后显著相关。胃癌组织中ME1表达与相邻组织中的ME1表达之间未发现显著差异。

结论

我们的结果提供了证据表明ME1是胃癌的关键因素。ME1在胃癌发生和迁移过程中可能是促癌的；它也可能与胃癌患者的生存有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ec9/6628973/c9b454a05771/OTT-12-5589-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ec9/6628973/7e33d0912709/OTT-12-5589-g0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ec9/6628973/6bc5b1131bdf/OTT-12-5589-g0005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ec9/6628973/c9b454a05771/OTT-12-5589-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ec9/6628973/7e33d0912709/OTT-12-5589-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ec9/6628973/9fc622a7d84d/OTT-12-5589-g0002.jpg
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