Qin Qiaoji, Lin Nan, Huang Huan, Zhang Xuezhi, Cao Xuelei, Wang Yongbin, Li Peng
Emergency Department, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, People's Republic of China.
Diabetes Metab Syndr Obes. 2019 Jul 10;12:1091-1103. doi: 10.2147/DMSO.S208989. eCollection 2019.
Ginsenoside Rg1 (GS Rg1), as an important active substance of Panax ginseng, has been proven to have elaborate cardioprotective effects. The purpose of this study was to detect that GS Rg1 attenuates cardiac oxidative stress and inflammation in streptozotocin (STZ)-induced diabetic rats (DM). Cardiac function was assessed by heart rate and blood pressure. Markers relevant to myocardial oxidative stress and antioxidant capacity, and inflammatory reaction factors were detected. The mRNA and protein expression were detected by RT-qPCR and Western blot, respectively. GS Rg1 treatment significantly reduced the symptoms of cardiac hypertrophy and hypertension, and also decreased oxidative stress, inflammation response, NF-κB expression and NLRP3 inflammasome expression. GS Rg1 enhanced mitochondrial biogenesis by increasing PGC-1α, complex III and complex Ⅳ expression. GS Rg1 treatment significantly increased the expression of AMPK, Nrf2 and HO-1 in cardiac tissues. GS Rg1 exhibited protective effect against STZ-induced cardiac dysfunction, which is potentially associated with AMPK/Nrf2/HO-1 signal pathway.
人参皂苷Rg1（GS Rg1）作为人参的一种重要活性物质，已被证明具有精细的心脏保护作用。本研究的目的是检测GS Rg1减轻链脲佐菌素（STZ）诱导的糖尿病大鼠（DM）心脏氧化应激和炎症。通过心率和血压评估心脏功能。检测与心肌氧化应激、抗氧化能力相关的标志物以及炎症反应因子。分别通过RT-qPCR和蛋白质免疫印迹法检测mRNA和蛋白质表达。GS Rg1治疗显著减轻了心脏肥大和高血压症状，还降低了氧化应激、炎症反应、NF-κB表达和NLRP3炎性小体表达。GS Rg1通过增加PGC-1α、复合物III和复合物IV的表达来增强线粒体生物合成。GS Rg1治疗显著增加了心脏组织中AMPK、Nrf2和HO-1的表达。GS Rg1对STZ诱导的心脏功能障碍具有保护作用，这可能与AMPK/Nrf2/HO-1信号通路有关。
