源自脂多糖刺激的人骨髓单核细胞HBL-38细胞的天然人干扰素-γ。
Natural human interferon-gamma derived from lipopolysaccharide-stimulated human myelomonocytic HBL-38 cells.
作者信息
Ando S, Ohta T, Tanimoto T, Sano O, Yamauchi H, Andoh O, Torigoe K, Kurimoto M
机构信息
Fujisaki Institute, Hayashibara Biochemical Laboratories Incorporated, Okayama.
出版信息
Jpn J Cancer Res. 1988 Jun;79(6):757-65. doi: 10.1111/j.1349-7006.1988.tb02233.x.
A human myelomonocytic cell line, HBL-38 cells, propagated in vivo, spontaneously produced interferon (IFN)-gamma and IFN-alpha. Whereas hemmagglutinating virus of Japan (HVJ) enhanced the production of IFN-alpha, bacterial lipopolysaccharide (LPS) markedly enhanced the production of IFN-gamma. LPS could be replaced with lipid A. Furthermore, the enhancement of production of IFN-gamma by LPS was completely abolished by polymixin B. IFN-gamma derived from LPS-stimulated HBL-38 cells was purified to homogeneity and characterized. The apparent molecular weight, subspecies composition, amino acid sequence and glycosylated sites were in agreement with those of the product of normal human peripheral blood lymphocytes (PBL). These results indicate that the myelomonocytic HBL-38 cells, not a T-cell line, can also produce IFN-gamma identical to the product of normal human PBL.
一种在体内增殖的人骨髓单核细胞系HBL - 38细胞可自发产生γ干扰素（IFN -γ）和α干扰素（IFN -α）。日本血凝病毒（HVJ）可增强IFN -α的产生，而细菌脂多糖（LPS）则显著增强IFN -γ的产生。LPS可用脂质A替代。此外，多黏菌素B可完全消除LPS对IFN -γ产生的增强作用。对LPS刺激的HBL - 38细胞产生的IFN -γ进行了纯化并鉴定其特性。其表观分子量、亚类组成、氨基酸序列和糖基化位点与正常人外周血淋巴细胞（PBL）的产物一致。这些结果表明，骨髓单核细胞系HBL - 38细胞而非T细胞系也能产生与正常人PBL产物相同的IFN -γ。
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