Asada H, Tamura M, Kondo K, Dohi Y, Yamanishi K
Department of Virology, Osaka University, Japan.
J Gen Virol. 1988 Sep;69 ( Pt 9):2179-88. doi: 10.1099/0022-1317-69-9-2179.
Cytotoxic T lymphocytes (CTLs) were generated when spleen cells from mice infected with viruses causing haemorrhagic fever with renal syndrome were stimulated in vitro with syngeneic cells infected with viruses. These cytotoxic effector cells, with Lyt2+ L3T4- markers on their surface, demonstrated H-2 restriction. CTLs induced by Hantaan virus (76-118 strain) or Seoul virus (B-1 strain) showed cross-reactivity with infected target cells. Hantaan virus infection induced a higher CTLs response than Seoul virus infection, although the antibody responses to these two viruses and the replication of the two virus strains in athymic nude mice were not significantly different. Viral antigen detected with a monoclonal antibody reacting with nucleocapsid antigen was observed mainly in the cytoplasm of macrophages infected with Hantaan virus, but in the nucleus of cells infected with Seoul virus. The major viral antigens recognized by CTLs are discussed on the basis of these findings.
当用感染了导致肾综合征出血热病毒的小鼠的脾细胞，在体外与感染了病毒的同基因细胞一起刺激时，产生了细胞毒性T淋巴细胞（CTL）。这些细胞毒性效应细胞表面带有Lyt2 + L3T4 - 标记，表现出H - 2限制性。由汉坦病毒（76 - 118株）或汉城病毒（B - 1株）诱导的CTL与感染的靶细胞表现出交叉反应性。尽管对这两种病毒的抗体反应以及这两种病毒株在无胸腺裸鼠中的复制没有显著差异，但汉坦病毒感染诱导的CTL反应比汉城病毒感染更高。用与核衣壳抗原反应的单克隆抗体检测到的病毒抗原，主要在感染汉坦病毒的巨噬细胞的细胞质中观察到，但在感染汉城病毒的细胞的细胞核中观察到。基于这些发现，讨论了CTL识别的主要病毒抗原。
