Kariwa H, Kimura M, Yoshizumi S, Arikawa J, Yoshimatsu K, Takashima I, Hashimoto N
Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Arch Virol. 1996;141(12):2327-38. doi: 10.1007/BF01718634.
To understand the mode of persistent infection of Seoul virus in rodents, we examined the distribution of the virus genome and antibody production in infected rats. When 1-day-old rats were inoculated with the KI-83-262 strain, the S segment of viral genome was detected in sera, clots, lungs and kidneys from 3 to 184 days post inoculation (d.p.i.) by nested reverse transcriptase PCR. On the other hand, when 7-week-old rats were infected with this virus, viral genome was detected only in the lungs from 3 to 50 d.p.i. The neutralizing antibody titers of rats inoculated at 1-day of age were higher than those of rats inoculated at 7 weeks of age. In both age groups, however, the IgG avidity of antibody increased along with the course of infection. We found that urban rats (Rattus norvegicus) infected early in life harbored the virus for more than 6 months.
为了解汉城病毒在啮齿动物中的持续感染模式，我们检测了受感染大鼠体内病毒基因组的分布及抗体产生情况。用KI - 83 - 262株接种1日龄大鼠后，通过巢式逆转录聚合酶链反应在接种后3至184天（d.p.i.）的血清、血凝块、肺和肾中检测到病毒基因组的S片段。另一方面，当7周龄大鼠感染该病毒时，仅在接种后3至50天在肺中检测到病毒基因组。1日龄接种大鼠的中和抗体滴度高于7周龄接种大鼠。然而，在两个年龄组中，抗体的IgG亲和力均随感染进程而增加。我们发现，幼年时感染的城市大鼠（褐家鼠）携带该病毒超过6个月。
