Asada H, Tamura M, Kondo K, Okuno Y, Takahashi Y, Dohi Y, Nagai T, Kurata T, Yamanishi K
J Gen Virol. 1987 Jul;68 ( Pt 7):1961-9. doi: 10.1099/0022-1317-68-7-1961.
Adult athymic nude (BALB/c background) mice or inbred BALB/c mice were inoculated intraperitoneally with Hantaan virus (HV), and attempts were made to isolate the virus from brain, lung and spleen. Virus was isolated from the organs of BALB/c mice for only a short time after infection but was isolated from various organs of nude mice consistently for at least 84 days after infection. Viral antigen was also detected in various organs of nude mice for a long time after infection. The effects of adoptive transfer of immune serum or immune T cells from BALB/c to nude mice before or after virus inoculation were examined. Before transfer, the T cell fraction was treated with complement (C') (group 1), anti-L3T4 + C' (group 2), anti-Lyt1.2 + C' (group 3) or anti-Lyt2.2 + C' (group 4). When transferred before virus inoculation to test the effects on protection against infection, immune serum and T cells of groups 1, 2 and 4 were effective. When transferred after HV inoculation to test the effects on clearance of virus, group 1 was the most effective followed by group 2. These results suggest that humoral and cellular immunity both have roles in protection against HV infection, and that T cells possessing L3T4- Lyt2+ markers on the cell-surface are especially important for elimination of infectious virus in vivo.
将成年无胸腺裸鼠（BALB/c背景）或近交系BALB/c小鼠经腹腔接种汉坦病毒（HV），并尝试从脑、肺和脾脏中分离病毒。仅在感染后短时间内从BALB/c小鼠的器官中分离到病毒，但在感染后至少84天内持续从裸鼠的各种器官中分离到病毒。在感染后很长一段时间内，也在裸鼠的各种器官中检测到病毒抗原。检测了在病毒接种之前或之后将BALB/c小鼠的免疫血清或免疫T细胞过继转移到裸鼠的效果。在转移之前，T细胞组分用补体（C'）处理（第1组）、抗L3T4 + C'（第2组）、抗Lyt1.2 + C'（第3组）或抗Lyt2.2 + C'（第4组）。当在病毒接种之前转移以测试对感染的保护作用时，第1、2和4组的免疫血清和T细胞是有效的。当在接种HV之后转移以测试对病毒清除的效果时，第1组最有效，其次是第2组。这些结果表明，体液免疫和细胞免疫在抵抗HV感染中均起作用，并且细胞表面具有L3T4 - Lyt2 +标记的T细胞对于体内感染性病毒的清除尤为重要。
