Department of Chemistry , Capital Normal University , Beijing 100048 , People's Republic of China.
Anal Chem. 2019 Sep 3;91(17):11433-11439. doi: 10.1021/acs.analchem.9b02855. Epub 2019 Aug 13.
Due to the high oxidative potential of the hydroxyl radical (OH), the accumulation of OH in tissues can cause inflammation, such as that in arthritis. Therefore, the development of OH detection with high efficiency and sensitivity is important for the treatment of related diseases. In this work, a cypate-modified core-shell NaErF@NaLuF nanoprobe (csEr-Cy) was designed for detecting OH on the basis of a typical reaction between cypate and OH. The process resulted in the recovery of 654 nm upconversion luminescence emission of csEr because of a weakened inner filter effect (IFE) and Förster resonance energy transfer (FRET). The short-wavelength infrared (SWIR) emission at 1550 nm was not affected by OH addition, thus enabling interference-free detection. Density functional theory (DFT) calculations were performed to explain the underlying mechanism. With the SWIR signal used as a reference for OH detection, the csEr-Cy nanoprobe showed higher sensitivity and penetration than visible reference. This method was successfully used in mice for the diagnosis of arthritis in vivo. Our results provide novel insights into improving the sensitivity of nanoprobes for molecule detection and disease diagnosis.
由于羟基自由基 (OH) 具有很高的氧化能力，因此 OH 在组织中的积累会引起炎症，如关节炎。因此，开发高效灵敏的 OH 检测方法对于相关疾病的治疗非常重要。在这项工作中，基于 cypate 与 OH 的典型反应，设计了一种 cypate 修饰的核壳型 NaErF@NaLuF 纳米探针（csEr-Cy）用于检测 OH。该过程导致 csEr 的上转换发光发射恢复 654nm，这是由于内滤效应（IFE）和Förster 共振能量转移（FRET）减弱。添加 OH 不会影响 1550nm 的短波红外（SWIR）发射，从而实现无干扰检测。进行了密度泛函理论（DFT）计算以解释潜在的机制。利用 SWIR 信号作为 OH 检测的参考，csEr-Cy 纳米探针的灵敏度和穿透性均高于可见参考。该方法成功用于小鼠体内关节炎的诊断。我们的结果为提高纳米探针用于分子检测和疾病诊断的灵敏度提供了新的见解。
