Department of Chemistry, Capital Normal University , Beijing 100048, People's Republic of China.
Anal Chem. 2017 May 2;89(9):4986-4993. doi: 10.1021/acs.analchem.7b00306. Epub 2017 Apr 11.
Hydroxyl radical (OH) is an important marker of the progress of heavy metal induced oxidative stress. However, most reported probes and detection methods cannot meet the need of monitoring the OH concentration within the whole progress because of the limited linear range. Besides, a low detection limit, high sensitivity, and good selectivity were also required. In this study, an ultrahigh sensitivity multifunctional nanoprobe (ICG-modified NaLuF:Yb,Er) was developed to evaluate heavy metal induced oxidative stress by detecting OH concentration, with a colorimetric, upconversion luminescence, and photothermal stepped method. This method has a broad linear detection range, from 16 pM to 2 μM, and a low detection limit of 4 pM. Besides, the nanoprobe showed less response to ions, amino acids, biomolecules, and other radical oxygen species (HO and O) than OH. This highly selective, highly sensitive probe with a broad linear detection range has great potential utility for monitoring OH concentration in live hypatocyte within the progress of heavy metal induced oxidative stress, with probable in vivo applications in the future.
羟基自由基（OH）是重金属诱导氧化应激进展的重要标志物。然而，由于线性范围有限，大多数报道的探针和检测方法都无法满足监测整个过程中 OH 浓度的需求。此外，还需要低检测限、高灵敏度和良好的选择性。在本研究中，开发了一种超高灵敏度多功能纳米探针（ICG 修饰的 NaLuF：Yb，Er），通过比色法、上转换发光和光热分步法来评估重金属诱导的氧化应激，以检测 OH 浓度。该方法具有从 16 pM 到 2 μM 的宽线性检测范围和 4 pM 的低检测限。此外，与 OH 相比，纳米探针对离子、氨基酸、生物分子和其他活性氧物质（HO 和 O）的响应较小。这种具有高选择性、高灵敏度和宽线性检测范围的探针具有在重金属诱导的氧化应激过程中监测活肝细胞中 OH 浓度的巨大潜在应用价值，未来可能在体内得到应用。
