Yu Guangshun, Feng Na, Zhao Dan, Wang Hao, Jin Yi, Liu Dandan, Li Zhenhua, Yang Xinjian, Ge Kun, Zhang Jinchao
Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, 071002, China.
College of Medical Science, Hebei University, Baoding, 071002, China.
Sci China Life Sci. 2021 Mar;64(3):434-442. doi: 10.1007/s11427-019-1601-2. Epub 2020 Mar 30.
Excessive reactive oxygen species (ROS) would attack living cells and cause a series of oxidative stress related diseases, such as liver damage. Hydroxyl radicals (·OH) are currently known as one of the most toxic and harmful free radicals to organisms. Therefore, studies involving hydroxyl radicals have become important research topics in the fields of biology, biochemistry, and biomedicine. In addition, imaging of analytes using upconversion nanoparticles (UCNPs) possesses significant advantages over that using general fluorescent dyes or nanoparticles due to its high spatial resolution, reduced photodamage, and deep tissue penetration properties. Herein, we designed a highly selective and sensitive hydroxyl radical nanoprobe based on the luminescence resonance energy transfer between upconversion nanoparticles and methylene blue (MB). The concentration of ·OH could be determined by the fluorescence recovery of the UCNPs due to the oxidative damage of MB. Using this nanoprobe, the ·OH in living cells or in liver tissues could be monitored with high sensitivity and selectivity.
过量的活性氧（ROS）会攻击活细胞并引发一系列与氧化应激相关的疾病，如肝损伤。羟基自由基（·OH）是目前已知对生物体毒性最大、危害最严重的自由基之一。因此，涉及羟基自由基的研究已成为生物学、生物化学和生物医学领域的重要研究课题。此外，使用上转换纳米粒子（UCNPs）对分析物进行成像，由于其具有高空间分辨率、减少光损伤和深层组织穿透特性，相比使用普通荧光染料或纳米粒子具有显著优势。在此，我们基于上转换纳米粒子与亚甲蓝（MB）之间的发光共振能量转移设计了一种高选择性和高灵敏度的羟基自由基纳米探针。由于MB的氧化损伤，·OH的浓度可以通过UCNPs的荧光恢复来确定。使用这种纳米探针，可以高灵敏度和高选择性地监测活细胞或肝组织中的·OH。
