Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Gregor-Mendel-Straße 4, 85354 Freising, Germany.
Grupo Tecnología de Enzimas, Centro de Ingeniería Genética y Biotecnología (CIGB), Ave 31 entre 158 y 190, Apartado Postal 6162, Habana, 10600, Cuba.
Environ Microbiol. 2019 Nov;21(11):4151-4165. doi: 10.1111/1462-2920.14768. Epub 2019 Aug 29.
Acetic acid bacteria (AAB) are associated with plants and insects. Determinants for the targeting and occupation of these widely different environments are unknown. However, most of these natural habitats share plant-derived sucrose, which can be metabolized by some AAB via polyfructose building levansucrases (LS) known to be involved in biofilm formation. Here, we propose two LS types (T) encoded by AAB as determinants for habitat selection, which emerged from vertical (T1) and horizontal (T2) lines of evolution and differ in their genetic organization, structural features and secretion mechanism, as well as their occurrence in proteobacteria. T1-LS are secreted by plant-pathogenic α- and γ-proteobacteria, while T2-LS genes are common in diazotrophic, plant-growth-promoting α-, β- and γ-proteobacteria. This knowledge may be exploited for a better understanding of microbial ecology, plant health and biofilm formation by sucrase-secreting proteobacteria in eukaryotic hosts.
醋酸菌（AAB）与植物和昆虫有关。针对这些广泛不同环境的目标和占据的决定因素尚不清楚。然而，这些自然栖息地大多都有植物来源的蔗糖，一些 AAB 可以通过多果糖合成莱伏门冬酰胺蔗糖酶（LS）来代谢蔗糖，已知 LS 参与生物膜的形成。在这里，我们提出了两种由 AAB 编码的 LS 类型（T）作为栖息地选择的决定因素，它们分别来自垂直（T1）和水平（T2）进化线，在遗传组织、结构特征和分泌机制方面存在差异，并且在变形菌门中也有存在。T1-LS 由植物病原性 α-和 γ-变形菌分泌，而 T2-LS 基因在固氮、促进植物生长的 α-、β-和 γ-变形菌中很常见。这一知识可以被利用来更好地理解微生物生态学、植物健康以及真核宿主中蔗糖酶分泌的变形菌的生物膜形成。
