采用模拟人体消化和 Caco-2 细胞摄取模型评估羽衣甘蓝中硫代葡萄糖苷的命运和生物利用度。

Assessing the Fate and Bioavailability of Glucosinolates in Kale () Using Simulated Human Digestion and Caco-2 Cell Uptake Models.

机构信息

Department of Nutrition and Culinary Science , Hankyong National University , 327 Chungang-Ro , Anseong-Si , Kyonggi-do 17579 , Korea.

Department of Biological and Agricultural Engineering, Department of Food Science and Technology , University of California, Davis , One Shields Avenue , Davis , California 95616 , United States.

出版信息

J Agric Food Chem. 2019 Aug 28;67(34):9492-9500. doi: 10.1021/acs.jafc.9b03329. Epub 2019 Aug 14.

DOI:10.1021/acs.jafc.9b03329

PMID:31374175

Abstract

Glucosinolates and their hydrolysis products were characterized in fresh and in gastric and intestinal digesta of Dinosaur kale ( L var. DC). In fresh kale, glucoraphanin, sinigrin, gluconapin, gluconasturtiin, glucoerucin, glucobrasscin, and 4-methoxylglucobrassicin were identified. After 120 min of gastric digestion, the levels of glucoraphanin, sinigrin, and gluconapin decreased, and no glucoerucin or glucobrasscin was detected. However, a concomitant increase in the glucosinolate hydrolysis products allyl nitrile, 3-butenyl isothiocyanate, phenylacetonitrile, and sulforaphane was observed. This trend continued through intestinal digestion. After 120 min, the levels of allyl nitrile, 3-butenyl isothiocyanate, phenylacetonitrile, and sulforaphane were 88.19 ± 5.85, 222.15 ± 30.26, 129.17 ± 17.57, and 13.71 ± 0.62 pmol/g fresh weight, respectively. Intestinal digesta were then applied to Caco-2 cell monolayers to assess the bioavailability. After 6 h of incubation, no glucosinolates were detected and the percentage of total cellular uptake of the glucosinolate hydrolysis products ranged from 29.35% (sulforaphane) to 46.60% (allyl nitrile).

摘要

对羽衣甘蓝（L. var. DC）的新鲜样品及其胃和肠道消化物中的硫代葡萄糖苷及其水解产物进行了鉴定。在新鲜羽衣甘蓝中鉴定出了萝卜硫苷、黑芥子苷、葡萄糖苷、葡萄糖苷辣根、葡萄糖异硫氰酸酯、葡萄糖芸薹素和 4-甲氧基葡萄糖芸薹素。经过 120 分钟的胃消化，萝卜硫苷、黑芥子苷和葡萄糖苷的水平下降，且未检测到葡萄糖异硫氰酸酯或葡萄糖芸薹素。然而，观察到硫代葡萄糖苷水解产物丙烯腈、3-丁烯基异硫氰酸酯、苯乙腈和萝卜硫素的含量同时增加。这种趋势一直持续到肠道消化。120 分钟后，丙烯腈、3-丁烯基异硫氰酸酯、苯乙腈和萝卜硫素的含量分别为 88.19 ± 5.85、222.15 ± 30.26、129.17 ± 17.57 和 13.71 ± 0.62 pmol/g 鲜重。然后将肠道消化物应用于 Caco-2 细胞单层以评估生物利用度。孵育 6 小时后，未检测到硫代葡萄糖苷，且硫代葡萄糖苷水解产物的总细胞摄取率在 29.35%（萝卜硫素）至 46.60%（丙烯腈）之间。