Lautier M, Gillot B, Auffray Y, Castillo-Sanchez X, Boutibonnes P
Laboratoire de Génétique Microbienne, Université de Caen, France.
Mutagenesis. 1988 May;3(3):245-7. doi: 10.1093/mutage/3.3.245.
The lethal and mutagenic effects of various mutagens on three strains of Streptococcus lactis were investigated. Lethality studies demonstrated that S. lactis was relatively sensitive to UV irradiation, methyl methanesulphonate (MMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and, to a lesser extent, to ethyl methanesulphonate (EMS). A spontaneous derivative Lac-, which has lost a 37-Md plasmid, was slightly more resistant and much less mutable than the wild-type after UV irradiation. Although the three strains were strongly mutated by EMS for the genetic marker assayed (Rifr), an increase in the mutation frequency was also observed after MMS and MNNG treatments.
研究了各种诱变剂对三株乳酸链球菌的致死和诱变作用。致死性研究表明，乳酸链球菌对紫外线照射、甲基磺酸甲酯（MMS）和N-甲基-N'-硝基-N-亚硝基胍（MNNG）相对敏感，对甲基磺酸乙酯（EMS）的敏感性稍低。一种自发衍生的Lac-菌株丢失了一个37-Md质粒，在紫外线照射后比野生型菌株略有抗性且突变性低得多。尽管对于所检测的遗传标记（Rifr），三株菌株都被EMS强烈诱变，但在MMS和MNNG处理后也观察到突变频率增加。
