Anderson D G, McKay L L
J Bacteriol. 1983 Aug;155(2):930-2. doi: 10.1128/jb.155.2.930-932.1983.
A recombination-deficient mutant of Streptococcus lactis ML3 designated MMS36 was isolated on the basis of its sensitivity to methyl methanesulfonate. This mutant also displayed sensitivity to UV irradiation. The inability of MMS36 to mediate homologous recombination was demonstrated by transduction of plasmid-linked lactose fermenting ability but not chromosomally mediated streptomycin resistance.
一株乳酸链球菌ML3的重组缺陷型突变体,命名为MMS36,是基于其对甲磺酸甲酯的敏感性而分离得到的。该突变体对紫外线照射也表现出敏感性。通过转导质粒连接的乳糖发酵能力而非染色体介导的链霉素抗性,证明了MMS36无法介导同源重组。