Auffray Y, Boutibonnes P
Unité de Toxicologie Génétique, Université de Caen, France.
Mutagenesis. 1987 Mar;2(2):107-9. doi: 10.1093/mutage/2.2.107.
The lethal and mutagenic effects of u.v. light and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on Bacillus thuringiensis were investigated. Lethality studies demonstrated that B. thuringiensis was relatively sensitive to these agents. This bacterium was mutated at the rifampicin resistance marker by u.v. light and to a lesser extent by the direct acting alkylating agent MNNG. One mutant selected for its greater sensitivity to u.v. light expressed a higher frequency of mutagenesis after u.v. light treatment and appeared to be defective in an excision repair pathway. However, this mutant was only slightly mutable by MNNG in comparison with the wild-type strain. This unusual phenotype does not yet have a parallel among the radiation sensitive mutants described in other bacterial species.
研究了紫外线和N-甲基-N'-硝基-N-亚硝基胍（MNNG）对苏云金芽孢杆菌的致死和诱变作用。致死性研究表明，苏云金芽孢杆菌对这些试剂相对敏感。该细菌在利福平抗性标记处被紫外线诱变，而直接作用的烷基化剂MNNG对其诱变程度较小。选择的一个对紫外线更敏感的突变体在紫外线处理后表现出更高的诱变频率，并且似乎在切除修复途径中存在缺陷。然而，与野生型菌株相比，该突变体仅被MNNG轻微诱变。这种不寻常的表型在其他细菌物种中描述的辐射敏感突变体中尚无类似情况。
