Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt.
Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt.
Ecotoxicol Environ Saf. 2019 Nov 15;183:109467. doi: 10.1016/j.ecoenv.2019.109467. Epub 2019 Jul 30.
Mancozeb (MZ) is a widely used ethylene-bis-dithiocarbamate fungicide in agriculture causing hepatoxic and genotoxic effects in rats. Curcumin (CUR) has various pharmacological effects including antioxidant and anti-inflammatory properties. This study investigated the efficacy of CUR in mitigating MZ-induced hepatotoxicity and genotoxicity in rats. Twenty-four male rats were divided into four equal groups; group I (control) was given carboxymethyl cellulose, group II was orally administered CUR (100 mg/kg b.wt), group III was gavaged with MZ (750 mg/kg b.wt), and group IV was co-treated with MZ and CUR at the same doses daily for 10 weeks. As a result, the concurrent treatment with CUR and MZ minimized the increased levels of liver function markers in serum, lipid peroxidation, pro-inflammatory mediators and DNA damage parameters in liver. In addition, CUR administration improved the depleted markers of hepatic antioxidant status in MZ-treated rats. Moreover, CUR protected the liver against the histological alterations elicited by MZ exposure and also, reduced the immunopositive reactivity of pro-apoptotic p53 in cytoplasm of hepatocytes. The present findings suggest that CUR exerts a significant protective effect against MZ-induced hepatotoxicity and genotoxicity.
代森锰锌（MZ）是一种广泛应用于农业的乙撑双二硫代氨基甲酸盐类杀菌剂，可导致大鼠肝毒性和遗传毒性。姜黄素（CUR）具有多种药理作用，包括抗氧化和抗炎特性。本研究旨在探讨 CUR 对减轻 MZ 诱导的大鼠肝毒性和遗传毒性的疗效。将 24 只雄性大鼠随机分为 4 组，每组 6 只：第 I 组（对照组）给予羧甲基纤维素，第 II 组给予 CUR（100mg/kg 体重），第 III 组给予 MZ（750mg/kg 体重），第 IV 组给予 MZ 和 CUR 同时给药，剂量与前两组相同，连续给药 10 周。结果表明，CUR 与 MZ 同时给药可降低血清中肝功能标志物、肝脂质过氧化、促炎介质和 DNA 损伤参数的升高水平。此外，CUR 可改善 MZ 处理大鼠肝抗氧化状态耗竭的标志物。此外，CUR 可保护肝脏免受 MZ 暴露引起的组织学改变，并降低细胞质中促凋亡 p53 的免疫阳性反应性。本研究结果表明，CUR 对 MZ 诱导的肝毒性和遗传毒性具有显著的保护作用。
