使用斑马鱼（Danio rerio）模型评估2.5微米细颗粒物（PM 2.5）暴露后的毛细胞损伤和发育毒性。
Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models.
作者信息
Rhee Jihye, Han Eunjung, Nam Kuk Jin, Lim Kang Hyeon, Chan Rah Yoon, Park Saemi, Koun Soonil, Park Hae-Chul, Choi June
机构信息
Department of Otorhinolaryngology-Head and Neck Surgery, Veterans Health Service Medical Center, Seoul, Republic of Korea; Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Korea University Ansan Hospital, Seoul, Republic of Korea.
Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Korea University Ansan Hospital, Seoul, Republic of Korea; Laboratory of Neurodevelopmental Genetics, Graduate School of Medicine, Korea University, Seoul, Republic of Korea.
出版信息
Int J Pediatr Otorhinolaryngol. 2019 Nov;126:109611. doi: 10.1016/j.ijporl.2019.109611. Epub 2019 Jul 28.
OBJECTIVES
Particulate matter (PM) exposure has become one of the most serious problems. The aim of the present study was to evaluate the hair cell damage and possible developmental toxicity caused by PM exposure using a zebrafish model.
METHODS
Zebrafish embryos were exposed to various concentrations of PM Developmental toxicity was evaluated based on general morphology score (GMS) system and Panzica-Kelly score, and by measurement of body length and heart rate. To evaluate hair cell damage, the average number of total hair cells within four neuromasts exposed to various concentrations of PM was compared with that of the control group.
RESULTS
Morphological abnormalities evaluated by the GMS system and Panzica-Kelly score were rare and body length tended to be shorter in the PM-exposed groups. Heart rate decreased significantly in the PM-exposed group. Additionally, significant hair cell damage was observed after PM exposure. It was dose-dependent and more severe after a longer period exposure (10 dpf).
CONCLUSIONS
In zebrafish embryos, exposure of PM in the early stages of life decreased heart rate and caused significant hair cell damage in a dose-dependent manner.
目的
颗粒物（PM）暴露已成为最严重的问题之一。本研究的目的是使用斑马鱼模型评估PM暴露引起的毛细胞损伤和可能的发育毒性。
方法
将斑马鱼胚胎暴露于不同浓度的PM中。基于总体形态评分（GMS）系统和潘齐卡 - 凯利评分，并通过测量体长和心率来评估发育毒性。为了评估毛细胞损伤，将暴露于不同浓度PM的四个神经丘内的总毛细胞平均数量与对照组进行比较。
结果
通过GMS系统和潘齐卡 - 凯利评分评估的形态学异常很少见，并且暴露于PM的组体长往往较短。暴露于PM的组心率显著降低。此外，PM暴露后观察到明显的毛细胞损伤。它呈剂量依赖性，并且在较长时间暴露（10 dpf）后更严重。
结论
在斑马鱼胚胎中，生命早期暴露于PM会降低心率，并以剂量依赖性方式导致明显的毛细胞损伤。
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