Manjunatha Bangeppagari, Deekshitha B, Seo Eunseok, Kim Jeongju, Lee Sang Joon
Center for Biofluid and Biomimic Research, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea.
Gandhi Medical College, Secunderabad, Telangana 500003, India.
Aquat Toxicol. 2021 Jul 30;238:105928. doi: 10.1016/j.aquatox.2021.105928.
Contemporary research in epidemiology has found that being exposed to air pollution at an early stage of life has associations with both acute and chronic conditions of the multi-organs. Nevertheless, the reasons for this have yet to be fully explained. Because of this there is a need for a robust investigation into the damaging toxic influence of diesel particulate matter (PM) on living organisms. This study is aimed to investigate the developmental toxicity of PM by using zebrafish (Danio rerio) embryo/larvae as a disease model and to understand the toxicity effects of PM on ecological environment more thoroughly. This research demonstrates that being exposed to PM leads to a significant increase in mortality, effective developmental morphology, reductions in hatching rates and lower heart rates in zebrafish. Additionally, it leads to increases in the length of string heart, area of pericardium, and apoptosis, reduces the number of normal intersegmental vessels (ISVs) and motor neurons in the trunk region and liver formation defects in zebrafish embryos. Investigation employing a scanning electron microscope demonstrates that being exposed to PM leads to damage in zebrafish larvae skin cell layers. Histological analysis demonstrates that when these larvae are treated with PM then abnormalities occur in the neurons, liver, heart, gills, brain, and eyes, and remarkable increase in in the cellular/subcellular levels of organelle dissolution. These findings are useful to help us understand the pathophysiological influence of being exposed to PM on the multi-organ defects of zebrafish. More research into which particular elements that make up diesel pollution contribute to this toxicity is needed so that the dangers to development can be further analysed.
当代流行病学研究发现，生命早期暴露于空气污染与多器官的急性和慢性疾病有关。然而，其原因尚未得到充分解释。因此，有必要对柴油颗粒物（PM）对生物体的有害毒性影响进行深入调查。本研究旨在以斑马鱼（Danio rerio）胚胎/幼虫为疾病模型，研究PM的发育毒性，以便更全面地了解PM对生态环境的毒性作用。本研究表明，暴露于PM会导致斑马鱼死亡率显著上升、有效发育形态改变、孵化率降低和心率下降。此外，它还会导致斑马鱼胚胎的心管长度增加、心包面积增加和细胞凋亡，减少躯干区域正常节段间血管（ISV）和运动神经元的数量以及肝脏形成缺陷。采用扫描电子显微镜的研究表明，暴露于PM会导致斑马鱼幼虫皮肤细胞层受损。组织学分析表明，当这些幼虫用PM处理时，神经元、肝脏、心脏、鳃、脑和眼睛会出现异常，细胞器溶解的细胞/亚细胞水平显著增加。这些发现有助于我们了解暴露于PM对斑马鱼多器官缺陷的病理生理影响。还需要进一步研究构成柴油污染的哪些特定元素导致了这种毒性，以便进一步分析其对发育的危害。
