College of Pharmaceutical Science, Zhejiang University of Technology, 310014 Hangzhou, China.
College of Pharmaceutical Science, Zhejiang University of Technology, 310014 Hangzhou, China.
J Pharm Biomed Anal. 2019 Oct 25;175:112789. doi: 10.1016/j.jpba.2019.112789. Epub 2019 Jul 24.
A molecularly imprinted membrane (MIM) with vancomycin (VCM) as a template and other related different material membranes (organic nylon microporous membranes, polyvinylidene fluoride (PVDF) membranes, polypropylene membranes) as a support respectively were synthesized by surface imprinting for the selective absorption of VCM, which can be used for pretreatment biological samples and rapid determination of VCM. The optimal ratio of template and functional monomer was 1:4, which was simulate by Gaussian, and the results showed that PVDF MIM had the best selectivity of the other three synthesized membranes to be used in the experiment. The maximum load on PVDF MIM was 23.8μg/cm, and on non-molecularly imprinted membrane was 1.7μg/cm. Additionally, performance of PVDF MIM, including surface structure, adsorption capacity, selective adsorption capacity, were also examined in this work. VCM in sample was enriched and separated effectively by PVDF MIM, and was quantitatively analyzed significantly by UV. The optimum eluent was 0.1 mol/L KOH aqueous solution in methanol (v/v=1/4), which desorption time was around 20 min. Also, recovery rate, precision, stability, linearity and repeatability for the novel assay were investigated respectively. The results indicated that separation and enrichment of VCM in biological samples by VCM MIM was reliable. The development of the assay, across a range of analytical and separated techniques for which MIM appeared to be the best support, was giving rise to increased interest in the determination of antibiotics as speedy assay for the blood concentration in clinical.
分别以万古霉素（VCM）为模板和其他相关不同材料膜（有机尼龙微孔膜、聚偏二氟乙烯（PVDF）膜、聚丙烯膜）为支撑，通过表面印迹合成了具有万古霉素（VCM）选择性吸附功能的分子印迹膜（MIM），可用于预处理生物样品和快速测定 VCM。通过高斯模拟，模板和功能单体的最佳比例为 1:4，结果表明，PVDF MIM 对其他三种合成膜具有最好的选择性，可用于实验。PVDF MIM 的最大载药量为 23.8μg/cm，而非分子印迹膜的载药量为 1.7μg/cm。此外，还对 PVDF MIM 的性能，包括表面结构、吸附容量和选择性吸附容量进行了考察。PVDF MIM 可有效富集和分离样品中的 VCM，并通过 UV 进行定量分析。最佳洗脱液为甲醇（v/v=1/4）中的 0.1mol/L KOH 水溶液，洗脱时间约为 20min。还分别考察了该新方法的回收率、精密度、稳定性、线性和重复性。结果表明，VCM MIM 对生物样品中 VCM 的分离和富集是可靠的。MIM 作为最佳支撑物，应用于分析和分离技术，使得抗生素的测定作为临床血药浓度的快速检测方法引起了人们的极大兴趣。
