Zhao Yan-Lin, You Yu-Xin, Chen Yu-Lang, Zhang Ying, Du Yan, Tang Dao-Quan
Department of Pharmacy, Suining People's Hospital Affiliated to Xuzhou Medical University Suining 221202 China.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University 209 Tongshan Road Xuzhou 221004 Jiangsu Province China
RSC Adv. 2024 Mar 11;14(12):8353-8365. doi: 10.1039/d4ra00294f. eCollection 2024 Mar 6.
An innovative molecularly imprinted polymer membrane (MIPM) was prepared with polyvinylidene difluoride (PVDF) as the support, phenytoin (PHT) as the single template, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linking reagent, azobisisobutyronitrile as the initiator, and acetonitrile-dimethylformamide (1 : 1.5, v/v) as the porogen. These materials were characterized scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller measurements and X-ray photoelectron spectroscopy. Their adsorption performances were evaluated through a series of experiments including isothermal adsorption, kinetic adsorption, selective adsorption, adsorption-desorption, reusability, and preparation reproducibility. Additionally, the application was explored by investigating the extraction recovery of MIPMs towards PHT, phenobarbital (PHB) and lamotrigine (LTG) in different matrices including methanol, normal saline (NS), phosphate buffer solution (PBS) and plasma. The results showed that MIPMs with rough and porous surfaces were successfully constructed, which offered good preparation reproducibility, reusability and selectivity. The adsorption capacities of MIPMs towards PHT, PHB and LTG were 2.312, 2.485 and 2.303 mg g, respectively, while their corresponding imprinting factors were 8.538, 12.122 and 4.562, respectively. The adsorption equilibrium of MIPMs was achieved within 20 min at room temperature without stirring or ultrasonication. The extraction recoveries of MIPMs for PHT, PHB or LTG in methanol, NS and PBS were more than 80% with an RSD% value of less than 3.64. In the case of plasma, the extraction recovery of MIPMs for PHT and PHB was more than 80% with an RSD% value of less than 2.41, while that of MIPMs for LTG was more than 65% with an RSD% value of less than 0.99. All the results indicated that the preparation method for MIPMs was simple, stable, and reliable, and the prepared MIPMs possessed excellent properties to meet the extraction application of PHT, PHB and LTG in different matrices.
以聚偏氟乙烯（PVDF）为载体，苯妥英（PHT）为单一模板，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂，偶氮二异丁腈为引发剂，乙腈 - 二甲基甲酰胺（1∶1.5，v/v）为致孔剂，制备了一种创新的分子印迹聚合物膜（MIPM）。通过扫描电子显微镜、傅里叶变换红外光谱、布鲁诺尔 - 埃米特 - 泰勒测量和X射线光电子能谱对这些材料进行了表征。通过一系列实验评估了它们的吸附性能，包括等温吸附、动力学吸附、选择性吸附、吸附 - 解吸、可重复使用性和制备重现性。此外，通过研究MIPM对甲醇、生理盐水（NS）、磷酸盐缓冲溶液（PBS）和血浆等不同基质中PHT、苯巴比妥（PHB）和拉莫三嗪（LTG）的萃取回收率，探索了其应用。结果表明，成功构建了具有粗糙多孔表面的MIPM，其具有良好的制备重现性、可重复使用性和选择性。MIPM对PHT、PHB和LTG的吸附容量分别为2.312、2.485和2.303 mg/g，而它们相应的印迹因子分别为8.538、12.122和4.562。在室温下，无需搅拌或超声处理，MIPM在20分钟内即可达到吸附平衡。MIPM对甲醇、NS和PBS中PHT、PHB或LTG的萃取回收率超过80%，相对标准偏差（RSD%）值小于3.64。在血浆中，MIPM对PHT和PHB的萃取回收率超过80%，RSD%值小于2.41，而MIPM对LTG的萃取回收率超过65%，RSD%值小于0.99。所有结果表明，MIPM的制备方法简单、稳定且可靠，所制备的MIPM具有优异的性能，能够满足不同基质中PHT、PHB和LTG的萃取应用。
