Department of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou 510642, PR China.
Department of Plant Pathology/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, PR China.
Food Chem. 2019 Dec 15;301:125224. doi: 10.1016/j.foodchem.2019.125224. Epub 2019 Jul 23.
This study was the first investigation into the main inducers of two lignifications by examining the changes of physicochemical properties and gene expression in king oyster mushrooms, under different conditions, during 21 days of storage. The results showed that the toughness, firmness and gumminess of the no-wounding treatment decreased to approximately 75-82.5% of the initial values, and the lignin content and expression of Pe4CL1 and Pe4CL3 decreased by 21-40% and 22-77%, respectively, in comparison to those of the other treatments in the first lignification. These findings indicated that wounding was the main factor inducing the first lignification. The second lignification of tested mushrooms was positively correlated with reactive oxygen species (ROS)-mediated senescence, accompanied by increased malondialdehyde (MDA) content, electrolyte leakage rate and mitochondrial dysfunction, which showed that ROS-mediated senescence played an essential role in the second lignification. This study is helpful for effective strategies to reduce lignification in stored mushrooms.
本研究首次通过在 21 天的贮藏过程中，在不同条件下研究大王蚝蘑中两种木质素形成的主要诱导因子，探讨其理化性质和基因表达的变化。结果表明，未损伤处理的韧性、硬度和胶粘性下降至初始值的 75-82.5%，木质素含量和 Pe4CL1、Pe4CL3 的表达量分别下降了 21-40%和 22-77%，与其他处理相比，在第一次木质素形成中。这些发现表明，损伤是诱导第一次木质素形成的主要因素。测试蘑菇的第二次木质素形成与活性氧（ROS）介导的衰老呈正相关，伴随着丙二醛（MDA）含量、电解质泄漏率和线粒体功能障碍的增加，表明 ROS 介导的衰老在第二次木质素形成中起着重要作用。本研究有助于为减少贮藏蘑菇中的木质素形成提供有效的策略。
