Universidad de Málaga, Andalucía Tech, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, E-29071 Málaga, Spain.
IBIMA (Biomedical Research Institute of Málaga), E-29071 Málaga, Spain.
Biomolecules. 2019 Aug 1;9(8):332. doi: 10.3390/biom9080332.
Endothelial cells form the inner lining of blood vessels, in a process known as angiogenesis. Excessive angiogenesis is a hallmark of several diseases, including cancer. The number of studies in endothelial cell metabolism has increased in recent years, and new metabolic targets for pharmacological treatment of pathological angiogenesis are being proposed. In this work, we wanted to address experimental evidence of substrate (namely glucose, glutamine and palmitate) dependence in immortalized dermal microvascular endothelial cells in comparison to primary endothelial cells. In addition, due to the lack of information about lactate metabolism in this specific type of endothelial cells, we also checked their capability of utilizing extracellular lactate. For fulfilling these aims, proliferation, migration, Seahorse, substrate uptake/utilization, and mRNA/protein expression experiments were performed. Our results show a high glycolytic capacity of immortalized dermal microvascular endothelial cells, but an early independence of glucose for cell growth, whereas a total dependence of glutamine to proliferate was found. Additionally, in contrast with reported data in other endothelial cell lines, these cells lack monocarboxylate transporter 1 for extracellular lactate incorporation. Therefore, our results point to the change of certain metabolic features depending on the endothelial cell line.
内皮细胞形成血管的内层，这个过程被称为血管生成。血管生成过度是包括癌症在内的几种疾病的标志。近年来，内皮细胞代谢的研究数量有所增加，并且正在提出针对病理性血管生成的药理学治疗的新代谢靶标。在这项工作中，我们希望针对与原代内皮细胞相比，永生化皮肤微血管内皮细胞对基质（即葡萄糖、谷氨酰胺和棕榈酸）的依赖性的实验证据进行研究。此外，由于缺乏关于这种特定类型内皮细胞中乳酸代谢的信息，我们还检查了它们利用细胞外乳酸的能力。为了实现这些目标，进行了增殖、迁移、 Seahorse 实验、基质摄取/利用以及 mRNA/蛋白表达实验。我们的结果表明，永生化皮肤微血管内皮细胞具有很高的糖酵解能力，但对葡萄糖的早期生长独立性，而发现完全依赖谷氨酰胺来增殖。此外，与其他内皮细胞系中的报道数据相反，这些细胞缺乏用于细胞外乳酸摄取的单羧酸转运蛋白 1。因此，我们的结果表明，某些代谢特征会根据内皮细胞系而发生变化。
