University of the Philippines - Philippine General Hospital, Department of Pediatrics, Philippines.
National Institute of Molecular Biology and Biotechnology, National Institutes of Health, University of the Philippines Manila, Philippines.
J Microbiol Immunol Infect. 2020 Oct;53(5):739-745. doi: 10.1016/j.jmii.2018.12.016. Epub 2019 Jul 17.
Hospital-care workers (HCWs) are at risk for MRSA carriage, subsequent infection and potential transmission of nosocomial infection. Epidemiological typing of MRSA among HCWs would provide data that can be used for control measures.
This is a cross sectional study that involved 92 participants from pediatric and surgery department of a tertiary hospital. Nasal swabs were collected and inoculated onto MRSASelect Chromogenic Media. Samples characterized as MRSA underwent SCCmec typing and detection of Panton Valentine leucocidin (PVL) by PCR.
The overall prevalence of MRSA was 13%. Six were from Pediatrics and another six were from Surgery. Seven out of 12 MRSA isolates carried SCCmec type I gene and five isolates carried SCCmec type IV gene. Six samples were found positive for PVL, four of which PVL-SSCmec IV, while the other two isolates were PVL-SCCmec I. The isolates were grouped into four main sequence types (STs) namely ST 1147, ST30, ST5 and ST97. Two samples from both departments were found to be PVL-positive SCCmec I ST 30; PVL-positive SCCmec IV ST 97 was found in two MRSA samples from Pediatrics and PVL-positive SCCmec IV ST 30 from Surgery.
Data collected from a non-outbreak setting suggest the presence of different clones of MRSA from nasal swabs of HCWs belonging to the Department of Pediatrics and Surgery. The data collected by this study can be used as reference for other succeeding studies on the surveillance of MRSA among HCWs.
医院工作人员（HCWs）有携带耐甲氧西林金黄色葡萄球菌（MRSA）、随后感染和潜在传播医院获得性感染的风险。对 HCWs 中 MRSA 的流行病学分型将提供可用于控制措施的数据。
这是一项横断面研究，涉及一家三级医院儿科和外科的 92 名参与者。采集鼻拭子并接种于 MRSASelect 显色培养基。特征为 MRSA 的样本进行 SCCmec 型分型和 Panton Valentine 白细胞毒素（PVL）的 PCR 检测。
MRSA 的总流行率为 13%。6 例来自儿科，6 例来自外科。12 株 MRSA 分离株中有 7 株携带 SCCmec 型 I 基因，5 株携带 SCCmec 型 IV 基因。6 个样本检测到 PVL 阳性，其中 4 个为 PVL-SCCmec IV，另外 2 个为 PVL-SCCmec I。分离株分为 4 个主要序列型（ST），即 ST1147、ST30、ST5 和 ST97。两个来自两个科室的样本被发现为 PVL 阳性 SCCmec I ST30；在儿科的 2 株 MRSA 样本中发现了 PVL 阳性 SCCmec IV ST97，在外科的 1 株 MRSA 样本中发现了 PVL 阳性 SCCmec IV ST30。
从非暴发环境中收集的数据表明，来自儿科和外科 HCWs 的鼻拭子中存在不同克隆的 MRSA。本研究收集的数据可作为其他 HCWs 中 MRSA 监测研究的参考。
