Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Microb Drug Resist. 2018 Dec;24(10):1543-1551. doi: 10.1089/mdr.2018.0033. Epub 2018 Jun 12.
The spread of Panton-Valentine leukocidin (PVL)-carrying strains in both hospital and the community is a significant worldwide problem. The aim of the study was to investigate the clonal dissemination pattern of PVL-producing strains isolated from hospitalized patients in Tehran, Iran. In this cross-sectional study, 70 PVL-carrying strains were recovered from 240 clinical specimens and characterized by antibiotic susceptibility testing, typing, SCC typing, typing, multilocus sequence typing, and virulence and adhesion gene profiling. All the PVL-carrying strains were confirmed as methicillin-resistant (MRSA) and recovered from wounds (48.6%), blood (25.7%), exudate/pus (11.4%), sputum (8.6%), and body fluid (5.7%) samples. Among the 70 PVL-carrying strains tested, 38 (54.3%) were positive for gene, 27 (38.6%) for , 13 (18.6%) for (A), 13 (18.6%) for (C) 13 (18.6%) for (M) 11 (15.7%) for (A) 10(14.3%) for (B), 9 (12.9%) for , 5 (7.1%) for , and 2 (2.9%) for (B) genes. Five clonal complexes (CC) and nine different clones were detected in this study. The most frequent CC was CC22 (ST22) (42.8%) followed by CC30 (ST30) (21.5%), CC8 (ST8) (17.2%), CC1 (ST772) (11.4%), and CC80 (ST80) (7.1%). In this study, ST22-SCC IV/t852 was the predominant PVL-positive MRSA clone (20%), followed by ST8-SCC IV/t008 (17.2%), ST30-SCC IV/t019 (12.9%), ST22-SCC IV/t790 (11.4%), ST22-SCC IV/t005 (11.4%), ST30-SCC IV/t021 (8.6%), ST80-SCC IV/t044 (7.1%), ST772-SCC V/t657 (7.1%), and ST772-SCC V/t10795 (4.3%). Diversity in clonal types of PVL-carrying MRSA strains in our study supports the need to perform a systematic surveillance of PVL-positive MRSA strains.
泛耐药性金黄色葡萄球菌（PVL）携带株在医院和社区的传播是一个全球性的重大问题。本研究旨在调查分离自伊朗德黑兰住院患者的产 PVL 菌株的克隆传播模式。在这项横断面研究中，从 240 份临床标本中回收了 70 株 PVL 携带株，并通过抗生素药敏试验、分型、SCC 分型、spa 分型、多位点序列分型以及毒力和黏附基因谱进行了特征分析。所有 PVL 携带株均确认为耐甲氧西林金黄色葡萄球菌（MRSA），并从伤口（48.6%）、血液（25.7%）、渗出物/脓液（11.4%）、痰（8.6%）和体液（5.7%）样本中分离出来。在 70 株检测的 PVL 携带株中，38 株（54.3%）携带 基因，27 株（38.6%）携带 基因，13 株（18.6%）携带 （A），13 株（18.6%）携带 （C），13 株（18.6%）携带 （M），11 株（15.7%）携带 （A），10 株（14.3%）携带 （B），9 株（12.9%）携带 基因，5 株（7.1%）携带 基因，2 株（2.9%）携带 （B）基因。本研究共检测到 5 个克隆复合体（CC）和 9 个不同的克隆。最常见的 CC 是 CC22（ST22）（42.8%），其次是 CC30（ST30）（21.5%）、CC8（ST8）（17.2%）、CC1（ST772）（11.4%）和 CC80（ST80）（7.1%）。在本研究中，ST22-SCC IV/t852 是主要的 PVL 阳性 MRSA 克隆（20%），其次是 ST8-SCC IV/t008（17.2%）、ST30-SCC IV/t019（12.9%）、ST22-SCC IV/t790（11.4%）、ST22-SCC IV/t005（11.4%）、ST30-SCC IV/t021（8.6%）、ST80-SCC IV/t044（7.1%）、ST772-SCC V/t657（7.1%）和 ST772-SCC V/t10795（4.3%）。本研究中 PVL 阳性耐甲氧西林金黄色葡萄球菌菌株的克隆类型多样性支持需要对 PVL 阳性耐甲氧西林金黄色葡萄球菌菌株进行系统监测。
