磷脂跨人红细胞膜外向转运的测量。
Measurement of outward translocation of phospholipids across human erythrocyte membrane.
作者信息
Bitbol M, Devaux P F
机构信息
Institute de Biologie Physico-Chimique, Paris, France.
出版信息
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6783-7. doi: 10.1073/pnas.85.18.6783.
Abstract
Spin-labeled phospholipids have been used to study the outside----inside and inside----outside transport of phospholipids across the human erythrocyte membrane at 37 degrees C. As already shown, inward transport is much faster for aminophospholipids than for phosphatidylcholine. In addition, we show here that outward transport of the phosphatidylserine and phosphatidylethanolamine analogues is three to four times faster than that of phosphatidylcholine. Magnesium depletion of the erythrocytes considerably decreases the outward rate of both aminophospholipids to values close to that of phosphatidylcholine. These results suggest that the outward aminophospholipid translocation is, at least partly, protein mediated. The protein involved could be identical to the inward Mg-ATP-dependent aminophospholipid carrier.
摘要
自旋标记的磷脂已被用于研究在37摄氏度下磷脂跨人红细胞膜的外向-内向和内向-外向转运。如已表明的那样，氨基磷脂的内向转运比磷脂酰胆碱快得多。此外，我们在此表明，磷脂酰丝氨酸和磷脂酰乙醇胺类似物的外向转运比磷脂酰胆碱快三到四倍。红细胞的镁缺乏会使两种氨基磷脂的外向转运速率大幅降低，降至接近磷脂酰胆碱的水平。这些结果表明，外向氨基磷脂易位至少部分是由蛋白质介导的。所涉及的蛋白质可能与内向Mg-ATP依赖性氨基磷脂载体相同。
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本文引用的文献
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