Dvision of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju, 52828, South Korea.
Department of Plant Medicine and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju, 52828, South Korea.
World J Microbiol Biotechnol. 2019 Aug 2;35(8):128. doi: 10.1007/s11274-019-2704-z.
Large patch disease, caused by Rhizoctonia solani AG2-2, is the most devastating disease in Zoysiagrass (Zoysia japonica). Current large patch disease control strategies rely primarily upon the use of chemical pesticides. Streptomyces sp. S8 is known to possess exceptional antagonistic properties that could potentially suppress the large patch pathogen found at turfgrass plantations. This study aims to demonstrate the feasibility of using the strain as a biological control mechanism. Sequencing of the S8 strain genome revealed a valinomycin biosynthesis gene cluster. This cluster is composed of the vlm1 and vlm2 genes, which are known to produce antifungal compounds. In order to verify this finding for the large patch pathogen, a valinomycin biosynthesis knockout mutant was created via the CRISPR/Cas9 system. The mutant lost antifungal activity against the large patch pathogen. Consequently, it is anticipated that eco-friendly microbial preparations derived from the S8 strain can be utilized to biologically control large patch disease.
丝核菌属 AG2-2 引起的大斑块病是结缕草（结缕草）最具破坏性的病害。目前大斑块病的防治策略主要依赖于化学农药的使用。链霉菌属 S8 具有特殊的拮抗特性，有可能抑制草坪植物上的大斑块病原体。本研究旨在证明该菌株作为生物防治机制的可行性。S8 菌株基因组的测序揭示了一个缬氨霉素生物合成基因簇。该簇由 vlm1 和 vlm2 基因组成，已知它们能产生抗真菌化合物。为了验证这一发现对大斑块病原体的作用，通过 CRISPR/Cas9 系统创建了缬氨霉素生物合成敲除突变体。突变体对大斑块病原体失去了抗真菌活性。因此，预计可以利用 S8 菌株衍生的环保微生物制剂来进行生物防治大斑块病。
