Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan.
Department of Environmental Sciences, University of Yamanashi, 4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.
Food Environ Virol. 2019 Dec;11(4):446-452. doi: 10.1007/s12560-019-09398-w. Epub 2019 Aug 2.
Five human-specific markers were detected in 59-74% of 27 human fecal-source samples collected in Yamanashi Prefecture, Japan. Similarly, potential human-specific markers, crAssphage, pepper mild mottle virus (PMMoV), and tobacco mosaic virus were detected in 96-100% of samples, with crAssphage showing the maximum concentration of 12.03 log copies/L. However, these markers were detected in 100% (3/3) of pig fecal-source samples, suggesting their applicability as general fecal pollution markers. Microbial source tracking analysis demonstrated that the rivers are contaminated by human and pig fecal sources. CrAssphage showed higher marker concentrations in river water samples than PMMoV, suggesting the preference of crAssphage to PMMoV as a marker of fecal pollution.
在日本山梨县采集的 27 个人类粪便样本中，有 59-74%检测到了 5 个人类特异性标志物。同样，在 100%（3/3）的猪粪便样本中也检测到了潜在的人类特异性标志物 crAssphage、辣椒轻斑驳病毒（PMMoV）和烟草花叶病毒，其中 crAssphage 的浓度最高，为 12.03 log 拷贝/L。这些标志物在 96-100%的样本中被检测到，表明它们可作为一般粪便污染标志物使用。微生物来源追踪分析表明，这些河流受到人类和猪粪便的污染。crAssphage 在河水样本中的标记浓度高于 PMMoV，表明 crAssphage 对 PMMoV 作为粪便污染标记物的偏好。
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