Dehghani-Samani Azam, Madreseh-Ghahfarokhi Samin, Dehghani-Samani Amir, Pirali Yaser
Faculty of Veterinary Medicine, Shahrekord University; Rahbar Boulevard, Shahrekord, Iran
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad; Azadi Square, Mashhad, Iran
Ann Parasitol. 2019;65(2):129-138. doi: 10.17420/ap6502.192.
In order to finding of potent natural medication/agent which can kill giardial cysts in the interval between lysing of outer membrane of cysts due to exposure by acidic condition of stomach and their encystation in proximal small intestine, In-vitro antigiardial activity and GC-MS analysis of Eucalyptus globulus and Zingiber officinalis against Giardia lamblia were studied in simulated condition to human’s body singly and in combination. Essential oils were extracted and their chemical components were identified via GC-MS method. After purification, cysts were exposure to acidic condition and were challenged by different concentrations of essential oils in simulated condition to human’s body. Percentages of inactivated (killed) cysts as efficacy of antigiardial activity were calculated and analyzed statistically. Presence of 1,8-eucalyptol, α-pinene, α-terpineol acetate, etc. in essential oil of E. globulus and presence of geraniol, α-zingiberene, (E,E)-α-farnesene, etc. in Z. officinalis essential oil were identified. Highest antigiardial activity (73.55%) was observed for eucalyptus essential oil in time 480 minutes after exposure. Efficacies of ginger and combined essential oils were different in different times. This study shows considerable antigiardial activity for both of essential oils singly and in combination together against giardial cysts. In-vivo study of protective effect of these essential oils against giardiasis can be considered as a subject for next studies.
为了找到有效的天然药物/药剂，能够在由于胃部酸性环境导致包囊外膜溶解与包囊在近端小肠内形成包囊的间隔期间杀死贾第虫包囊，在模拟人体条件下单独及联合研究了蓝桉和姜对贾第鞭毛虫的体外抗贾第虫活性及气相色谱-质谱（GC-MS）分析。提取了精油并通过GC-MS方法鉴定其化学成分。纯化后，将包囊暴露于酸性条件下，并在模拟人体条件下用不同浓度的精油进行挑战。计算并统计分析灭活（杀死）包囊的百分比作为抗贾第虫活性的功效。鉴定出蓝桉精油中存在1,8-桉叶素、α-蒎烯、α-松油醇乙酸酯等，姜精油中存在香叶醇、α-姜烯、（E,E）-α-法呢烯等。暴露480分钟后，观察到桉叶精油的抗贾第虫活性最高（73.55%）。姜精油和混合精油的功效在不同时间有所不同。本研究表明，这两种精油单独及联合使用对贾第虫包囊均具有显著的抗贾第虫活性。这些精油对贾第虫病保护作用的体内研究可作为后续研究的课题。
