CD44基因rs8193 C等位基因在胃癌患者中显著富集。
CD44 Gene rs8193 C Allele Is Significantly Enriched in Gastric Cancer Patients.
作者信息
Mokhtarian Roya, Tabatabaeian Hossein, Saadatmand Pardis, Azadeh Mansoureh, Balmeh Negar, Yakhchali Bagher, Ghaedi Kamran
机构信息
Division of Cellular and Molecular Biology, Department of Biology, NourDanesh Institute of Higher Education, Meymeh, Iran.
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
出版信息
Cell J. 2020 Jan;21(4):451-458. doi: 10.22074/cellj.2020.6389. Epub 2019 Jul 29.
OBJECTIVE
Gastric cancer is a multifactorial disease. In addition to environmental factors, many genes are involved in this malignancy. One of the genes associated with gastric cancer is gene and its polymorphisms. gene plays role in regulating cell survival, growth and mobility. The single nucleotide polymorphism (SNP) rs8193, located in the gene, has not been studied in gastric cancer patients of the Iranian population. The present study aims to study this polymorphism in 86 gastric cancer patients and 96 healthy individuals.
MATERIALS AND METHODS
In this cross-sectional case-control study, rs8193 polymorphism was genotyped by allele specific primer polymerase chain reaction (ASP-PCR) technique. The obtained data were statistically analyzed. To find the potential mechanism of action, rs8193 was bioinformatically investigated.
RESULTS
rs8193 C allele played a risk factor role for gastric cancer. Patients carrying this allele were more susceptible to have gastric cancer, with lymph node spread. On the other hand, rs8193 T allele, a protective factor, was associated with a higher chance of accumulation in the lower stages of cancer. C allele might impose its effect via destabilizing and miR-570 interaction.
CONCLUSION
rs8193 is statistically associated with the risk of malignancy, lymph node spread and stage of gastric cancer in Iranian population.
目的
胃癌是一种多因素疾病。除环境因素外，许多基因也参与了这种恶性肿瘤的发生。与胃癌相关的基因之一是[具体基因名称]基因及其多态性。[具体基因名称]基因在调节细胞存活、生长和迁移中发挥作用。位于[具体基因名称]基因中的单核苷酸多态性（SNP）rs8193，尚未在伊朗人群的胃癌患者中进行研究。本研究旨在对86例胃癌患者和96名健康个体进行该多态性的研究。
材料与方法
在这项横断面病例对照研究中，采用等位基因特异性引物聚合酶链反应（ASP-PCR）技术对rs8193多态性进行基因分型。对获得的数据进行统计学分析。为了找出潜在的作用机制，对rs8193进行了生物信息学研究。
结果
rs8193 C等位基因对胃癌起危险因素作用。携带该等位基因的患者更容易患胃癌，并伴有淋巴结转移。另一方面，rs8193 T等位基因作为一种保护因素，与癌症较低分期的更高累积几率相关。C等位基因可能通过破坏[具体基因名称]和miR-570的相互作用来发挥其作用。
结论
在伊朗人群中，rs8193与胃癌的恶性风险、淋巴结转移及分期在统计学上相关。
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