Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, PR China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Poult Sci. 2019 Nov 1;98(11):6026-6033. doi: 10.3382/ps/pez414.
The expression of env proteins that bind to viral cell receptors on avian leukosis virus (ALV)-susceptible cells can block ALV infection. In this study, we constructed a cell line (DF-1/B) by expressing the ALV-B env protein in DF-1 cells. PCR, immune fluorescence assay, Western blot, and immune electron microscopy results showed that the env gene can be stably expressed in DF-1cells and the env protein could be detected on the DF-1 cell membrane. An antiviral experiment concluded that the DF-1/B cell line could be resistant to 1 × 104 TCID50 ALV-B virus infection, but had no inhibitory effect on other subgroup ALV. This means that the DF-1/B cell line is specifically resistant to ALV-B and can be used as a tool for ALV-B diagnosis.
在易感性禽白血病病毒（ALV）的细胞上表达与病毒细胞受体结合的 env 蛋白可阻止 ALV 感染。在本研究中，我们通过在 DF-1 细胞中表达 ALV-B env 蛋白构建了一个细胞系（DF-1/B）。PCR、免疫荧光检测、Western blot 和免疫电镜结果表明，env 基因可以在 DF-1 细胞中稳定表达，env 蛋白可以在 DF-1 细胞膜上检测到。抗病毒实验表明，DF-1/B 细胞系可抵抗 1×104 TCID50 ALV-B 病毒感染，但对其他亚群的 ALV 无抑制作用。这意味着 DF-1/B 细胞系对 ALV-B 具有特异性抗性，可作为 ALV-B 诊断的工具。
