Lhx6在成年及发育中小鼠视网膜中的表达。

Expression of Lhx6 in the adult and developing mouse retina.

作者信息

He Daqiang, Guo Rui, Zheng Dongwang, Li Ping, Dong Xuhui, Gan Lin

机构信息

College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China; Institute of Life Sciences, Hangzhou Normal University, Hangzhou, 310018, Zhejiang, China; Department of Laboratory Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, Zhejiang, China.

College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.

出版信息

Gene Expr Patterns. 2019 Dec;34:119065. doi: 10.1016/j.gep.2019.119065. Epub 2019 Jul 31.

DOI:10.1016/j.gep.2019.119065

PMID:31376504

Abstract

PURPOSE

To investigate the expression patterns of LIM Homeobox 6 (Lhx6) in the adult and developing mouse retina.

METHODS

The Lhx6-GFP knock-in allele was used to activate constitutive expression of a GFP reporter in Lhx6 expressing cells. Double labeling with GFP and retinal markers in the mouse retina at postnatal day 56 (P56) was performed to identify the cell types expressing Lhx6. To determine the neuronal cell types that express Lhx6, double labeling with GFP and various retinal markers was employed in the differentiating retina at P7 and P15.

RESULTS

GFP + Lhx6 lineage cells were determined in Brn3a + retinal ganglion cells (RGCs), ChAT + amacrine cells (ACs), and Islet-class LIM-homeodomain 1 (Isl1+) ACs in the mouse retina at P56. In the ganglion cell layer (GCL), Lhx6 was expressed in Brn3a + RGCs but not Brn3b + RGCs at P15. Moreover, in the inner nuclear layer (INL), Lhx6 was not expressed in Bhlhb5+ ACs at P15. However, Lhx6 was weakly expressed in Glyt1+ ACs and Pax6+ ACs, and strongly expressed in Isl1+ and ChAT + ACs at P15.

CONCLUSION

Lhx6 was expressed in RGCs and ACs in both the adult and developing mouse retina.

摘要

目的

研究LIM同源盒6（Lhx6）在成年和发育中小鼠视网膜中的表达模式。

方法

利用Lhx6-GFP基因敲入等位基因激活Lhx6表达细胞中GFP报告基因的组成性表达。在出生后第56天（P56）的小鼠视网膜中进行GFP与视网膜标记物的双重标记，以鉴定表达Lhx6的细胞类型。为了确定表达Lhx6的神经元细胞类型，在P7和P15的分化视网膜中进行GFP与各种视网膜标记物的双重标记。

结果

在P56的小鼠视网膜中，在Brn3a+视网膜神经节细胞（RGCs）、ChAT+无长突细胞（ACs）和胰岛类LIM同源域1（Isl1+）ACs中确定了GFP+Lhx6谱系细胞。在神经节细胞层（GCL）中，P15时Lhx6在Brn3a+RGCs中表达，但在Brn3b+RGCs中不表达。此外，在P15时，在内核层（INL）中，Lhx6在Bhlhb5+ACs中不表达。然而，Lhx6在Glyt1+ACs和Pax6+ACs中弱表达，在P15时在Isl1+和ChAT+ACs中强表达。