Zhang Li, Huang Yuemeng, Wang Meng, Guo Yafen, Liang Jing, Yang Xiurong, Qi Wenjing, Wu Yanjun, Si Jinglei, Zhu Siran, Li Zhe, Li Ruiqiang, Shi Chao, Wang Shuo, Zhang Qunjie, Tang Zhonglin, Wang Lixian, Li Kui, Fei Ji-Feng, Lan Ganqiu
College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
College of Animal Science and Technology, Guangxi University, Nanning 530004, China; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
iScience. 2019 Sep 27;19:162-176. doi: 10.1016/j.isci.2019.07.025. Epub 2019 Jul 20.
Pig has been proved to be a valuable large animal model used for research on diabetic disease. However, their translational value is limited given their distinct anatomy and physiology. For the last 30 years, we have been developing a laboratory Asian miniature pig inbred line (Bama miniature pig [BM]) from the primitive Bama xiang pig via long-term selective inbreeding. Here, we assembled a BM reference genome at full chromosome-scale resolution with a total length of 2.49 Gb. Comparative and evolutionary genomic analyses identified numerous variations between the BM and commercial pig (Duroc), particularly those in the genetic loci associated with the features advantageous to diabetes studies. Resequencing analyses revealed many differentiated gene loci associated with inbreeding and other selective forces. These together with transcriptome analyses of diabetic pig models provide a comprehensive genetic basis for resistance to diabetogenic environment, especially related to energy metabolism.
猪已被证明是用于糖尿病疾病研究的有价值的大型动物模型。然而，鉴于其独特的解剖学和生理学特征，它们的转化价值有限。在过去30年里，我们通过长期选择性近亲繁殖，从原始的巴马香猪培育出了一个实验室亚洲小型猪近交系（巴马小型猪[BM]）。在此，我们以全染色体规模分辨率组装了一个BM参考基因组，全长2.49Gb。比较和进化基因组分析确定了BM和商业猪（杜洛克猪）之间的众多变异，特别是那些与有利于糖尿病研究的特征相关的基因座中的变异。重测序分析揭示了许多与近亲繁殖和其他选择力相关的分化基因座。这些与糖尿病猪模型的转录组分析一起，为抵抗致糖尿病环境，特别是与能量代谢相关的环境，提供了全面的遗传基础。
