Department of Chemistry and Nano Science, Ewha Womans University, Seoul 03760, Republic of Korea; Faculty of Science and Engineering, Meijo University, Nagoya, Aichi 468-8502, Japan.
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 03760, Republic of Korea; Research Institute for Basic Sciences, Ewha Womans University, Seoul 03760, Republic of Korea.
J Inorg Biochem. 2019 Oct;199:110777. doi: 10.1016/j.jinorgbio.2019.110777. Epub 2019 Jul 13.
A large number of industrially relevant enzymes depend upon dihydronicotinamide adenine dinucleotide (NADH) and dihydronicotinamide adenine dinucleotide phosphate (NADPH) cofactors, which are too expensive to be added in stoichiometric amounts. Existing NAD(P)H-recycling systems suffer from low activity, or the generation of side products. This review focuses on NAD(P)H cofactor regeneration catalyzed by transition metal complexes such as rhodium, ruthenium and iridium complexes using cheap reducing agents such as hydrogen (H) and ethanol, which have attracted increasing attention as sustainable energy carriers. The catalytic mechanisms for the regioselective reduction of NAD(P) are discussed with emphasis on identification of catalytically active intermediates such as transition metal hydride complexes. Applications of NAD(P)H-recycling systems to develop artificial photosynthesis are also discussed.
大量与工业相关的酶依赖于二氢烟酰胺腺嘌呤二核苷酸（NADH）和二氢烟酰胺腺嘌呤二核苷酸磷酸（NADPH）辅因子，但这些辅因子过于昂贵，无法以化学计量的方式添加。现有的 NAD(P)H 再生系统活性低，或者会产生副产物。本综述重点介绍了使用廉价还原剂（如氢气（H）和乙醇），由铑、钌和铱等过渡金属配合物催化的 NAD(P)H 辅因子再生，这些还原剂作为可持续能源载体越来越受到关注。讨论了 NAD(P)区域选择性还原的催化机制，重点是鉴定催化活性中间体，如过渡金属氢化物配合物。还讨论了 NAD(P)H 再生系统在开发人工光合作用中的应用。
