Hefei National Research Center for Physical Sciences at the Microscale, Collaborative Innovative Center of Chemistry for Energy Materials (iChEM), School of Chemistry and Materials Science, University of Science and Technology of China, 230026, Hefei, Anhui, China.
Institute of Energy, Hefei Comprehensive National Science Center, 350 Shushanhu Rd., 230031, Hefei, Anhui, China.
Nat Commun. 2023 Oct 25;14(1):6783. doi: 10.1038/s41467-023-42591-x.
Programmable artificial photosynthetic cell is the ultimate goal for mimicking natural photosynthesis, offering tunable product selectivity via reductase selection toward device integration. However, this concept is limited by the capacity of regenerating the multiple cofactors that hold the key to various reductases. Here, we report the design of artificial photosynthetic cells using biotic-abiotic thylakoid-CdTe as hybrid energy modules. The rational integration of thylakoid with CdTe quantum dots substantially enhances the regeneration of bioactive NADPH, NADH and ATP cofactors without external supplements by promoting proton-coupled electron transfer. Particularly, this approach turns thylakoid highly active for NADH regeneration, providing a more versatile platform for programming artificial photosynthetic cells. Such artificial photosynthetic cells can be programmed by coupling with diverse reductases, such as formate dehydrogenase and remodeled nitrogenase for highly selective production of formate or methane, respectively. This work opens an avenue for customizing artificial photosynthetic cells toward multifarious demands for CO conversion.
可编程人工光合作用细胞是模拟自然光合作用的终极目标，通过选择还原酶来实现可调产物选择性，从而实现器件集成。然而，这个概念受到了再生多种辅酶的能力的限制，而这些辅酶是各种还原酶的关键。在这里，我们报告了使用生物- 无机类囊体-CdTe 作为混合能量模块的人工光合作用细胞的设计。通过促进质子耦合电子转移，类囊体与 CdTe 量子点的合理整合极大地增强了生物活性 NADPH、NADH 和 ATP 辅酶的再生，而无需外部补充。特别是，这种方法使类囊体对 NADH 的再生具有高度活性，为人工光合作用细胞的编程提供了一个更通用的平台。通过与不同的还原酶（如甲酸脱氢酶和改造的固氮酶）偶联，这些人工光合作用细胞可以被编程，分别高度选择性地生产甲酸或甲烷。这项工作为定制人工光合作用细胞以满足 CO 转化的各种需求开辟了一条途径。
