Huang N, Lineberger B, Steiner M
Division of Hematology/Oncology, Memorial Hospital of R.I., Pawtucket.
Thromb Res. 1988 May 15;50(4):547-57. doi: 10.1016/0049-3848(88)90203-4.
The effect of alpha-tocopherol on various endothelial cell functions was evaluated in vitro using cultures of human umbilical vein endothelial cells. Prostacyclin synthesis, plasminogen activator activity and von Willebrand factor activity were evaluated in control and alpha-tocopherol supplemented cultures. Alpha-tocopherol produced stimulation of prostacyclin production which peaked at or near 0.5 mM. Plasminogen activator activity was markedly reduced by the addition of alpha-tocopherol. Von Willebrand factor activity showed a significant decrease of processed and cellular forms that was dose-dependent. The incorporation of alpha-tocopherol into endothelial cells could be demonstrated by measuring intracellular levels of the vitamin in washed endothelial cells. Our results show that alpha-tocopherol has a profound effect on multiple endothelial cell functions which are intimately involved in hemostasis.
利用人脐静脉内皮细胞培养物在体外评估了α-生育酚对各种内皮细胞功能的影响。在对照培养物和补充了α-生育酚的培养物中评估了前列环素合成、纤溶酶原激活物活性和血管性血友病因子活性。α-生育酚可刺激前列环素的产生，在0.5 mM或接近该浓度时达到峰值。添加α-生育酚可使纤溶酶原激活物活性显著降低。血管性血友病因子活性显示，其加工形式和细胞形式均呈剂量依赖性显著下降。通过测量洗涤后内皮细胞中维生素的细胞内水平，可以证明α-生育酚被内皮细胞摄取。我们的结果表明，α-生育酚对多种内皮细胞功能有深远影响，而这些功能与止血密切相关。
