Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, PR China; Beijing Key Laboratory of Radioactive Waste Treatment, INET, Tsinghua University, Beijing 100084, PR China.
School of Water Resources and Environment, China University of Geosciences, Beijing 100083, PR China.
Waste Manag. 2019 Aug 1;96:190-197. doi: 10.1016/j.wasman.2019.07.031. Epub 2019 Jul 25.
Ionizing radiation coupled with peroxymonosulfate (PMS) oxidation was developed to degrade antibiotics and antibiotic resistance genes (ARGs) from the erythromycin fermentation (EryF) residual wastes. The experimental results showed that the ERY content and ARGs abundance decreased with increase of the absorbed dose and PMS dosage and gamma irradiation was more effective to abate ARGs from the EryF wastes. The removal efficiency of ERY reached 49-55% and more than 96-99% of ARGs (1.32-2.55 log) was eliminated with the absorbed dose of 25-50 kGy and PMS dosage of 50-100 mM. Illumina pyrosequencing revealed that 3 bacterial phyla, Proteobacteria, Firmicutes and Fusobacteria were highly enriched and the ARGs-linked hosts were affiliated to the genera Aeromonas, Enterobacteriaceae and Enterobacter in the phylum Proteobacteria. The abundance of the ARGs-linked bacteria decreased by gamma/PMS treatment. Ionizing radiation/PMS treatment with the doses of 25 kGy and 50 mM PMS is proposed for potential practical application.
辐照与过一硫酸盐（PMS）氧化相结合，被开发用于降解红霉素发酵（EryF）剩余废物中的抗生素和抗生素抗性基因（ARGs）。实验结果表明，ERY 含量和 ARGs 丰度随吸收剂量和 PMS 剂量的增加而降低，γ 辐照对降低 EryF 废物中的 ARGs 更有效。吸收剂量为 25-50 kGy 和 PMS 剂量为 50-100 mM 时，ERY 的去除效率达到 49-55%，超过 96-99%的 ARGs（1.32-2.55 log）被消除。Illumina 焦磷酸测序显示，3 个细菌门，变形菌门、厚壁菌门和梭杆菌门高度富集，与 ARGs 相关的宿主隶属于变形菌门的气单胞菌属、肠杆菌科和肠杆菌属。辐照/PMS 处理后，与 ARGs 相关的细菌丰度降低。提出了 25 kGy 的辐照剂量和 50 mM PMS 的剂量用于潜在的实际应用。
