College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Biol Macromol. 2019 Oct 15;139:342-351. doi: 10.1016/j.ijbiomac.2019.07.195. Epub 2019 Aug 1.
Oligosaccharides from green algae Ulva lactuca (ULO) and Enteromorpha prolifera (EPO) were used for investigation of anti-ageing effects and the underlying mechanism in SAMP8 mice. The structural properties of ULO and EPO were analyzed by fourier-transform infrared spectroscopy, gas chromatography-mass spectrometry, and agarose gel electrophoresis. These oligosaccharides enhanced the glutathione, superoxide dismutase, catalase, and telomerase levels and total antioxidant capicity, and decreased the levels of malondialdehyde and advanced glycation end products. After ULO and EPO treatment, the levels of inflammatory factors, including IFN-γ, TNF-α, and IL-6, decreased; the BDNF and ChAT levels increased; and hippocampal neurons were protected. Downregulation of the p53 and FOXO1 genes and upregulation of the Sirt1 gene indicated that ULO and EPO have potential therapeutic effects in the prevention of ageing in SAMP8 mice. By 16S rRNA gene high-throughput sequencing, the abundance of Desulfovibrio was discovered to be markedly different in mice treated with ULO and EPO. The abundances of Verrucomicrobiaceae, Odoribacteraceae, Mogibacteriaceae, Planococcaceae, and Coriobacteriaceae were positively correlated with age-related indicators. These results demonstrated that oligosaccharides from U. lactuca and E. prolifera are ideal candidate compounds that can be used in functional foods and pharmaceuticals to prevent ageing.
绿藻石莼（ULO）和浒苔（EPO）中的低聚糖被用于研究 SAMP8 小鼠的抗衰老作用及其潜在机制。采用傅里叶变换红外光谱、气相色谱-质谱联用和琼脂糖凝胶电泳分析了 ULO 和 EPO 的结构特性。这些低聚糖可提高谷胱甘肽、超氧化物歧化酶、过氧化氢酶和端粒酶的水平以及总抗氧化能力，降低丙二醛和晚期糖基化终产物的水平。经 ULO 和 EPO 处理后，炎性因子 IFN-γ、TNF-α和 IL-6 的水平降低；BDNF 和 ChAT 水平升高；海马神经元得到保护。p53 和 FOXO1 基因下调，Sirt1 基因上调，表明 ULO 和 EPO 对 SAMP8 小鼠的衰老具有潜在的治疗作用。通过 16S rRNA 基因高通量测序发现，ULO 和 EPO 处理的小鼠中脱硫弧菌的丰度明显不同。疣微菌科、恶臭杆菌科、莫格利希菌科、棒状杆菌科和柯里伯氏菌科的丰度与年龄相关指标呈正相关。这些结果表明，U. lactuca 和 E. prolifera 中的低聚糖是理想的候选化合物，可用于功能性食品和药物，以预防衰老。
