Department of Breast Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, China.
Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, China.
Sleep Breath. 2024 Nov 28;29(1):18. doi: 10.1007/s11325-024-03193-z.
BACKGROUND/OBJECTIVE: Short sleep duration (SSD) affects people's health in multiple ways. This study attempted to explore the effect of SSD on the gut microbiota.
In the American Gut Project Database, 361 individuals (without troubled by disease recently) with less than 6 h of sleep per day were obtained and matched with normal sleep time individuals according to gender, age, and BMI. Furthermore, the raw data of 16s rRNA in feces were downloaded and analyzed using QIIME2, and STAMP was used for data statistics. PICRUST2 was used for predicting the alteration of microbial function.
The SSD did not affect the microbial α-diversity. SSD increased the abundance of the phylum Verrucomicrobia and the families Rikenellaceae, Verrucomicrobiaceae, and S24-7, and decrased the Coriobacteriaceae. Moreover, PICRUST2 predicted that SSD affected 15 metabolic pathways. Subgroup analyses showed that SSD had more significant effects on the microbiota in normal-weight females.
SSD substantially modifies the abundance of specific gut microbiota taxa, exerting a pronounced influence particularly on females, highlighting the need for further investigation into the bidirectional relationship between sleep patterns and gut microbiota.
背景/目的：睡眠不足（SSD）会以多种方式影响人们的健康。本研究试图探讨 SSD 对肠道微生物群的影响。
从美国肠道计划数据库中获取了 361 名每天睡眠少于 6 小时（近期未受疾病困扰）的个体，并根据性别、年龄和 BMI 与正常睡眠时间的个体进行匹配。此外，下载粪便 16s rRNA 的原始数据，并使用 QIIME2 进行分析，使用 STAMP 进行数据统计。使用 PICRUST2 预测微生物功能的改变。
SSD 不影响微生物的 α 多样性。SSD 增加了厚壁菌门和理研菌科、疣微菌科和 S24-7 科的丰度，并降低了科里氏杆菌科的丰度。此外，PICRUST2 预测 SSD 影响了 15 个代谢途径。亚组分析表明，SSD 对正常体重女性的微生物群有更显著的影响。
SSD 大量改变了特定肠道微生物群的丰度，对女性的影响尤为明显，这突显了进一步研究睡眠模式和肠道微生物群之间双向关系的必要性。
