National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China.
Bioresour Technol. 2019 Nov;291:121914. doi: 10.1016/j.biortech.2019.121914. Epub 2019 Jul 27.
Dynamics of nitrification activity, ammonia-oxidizing archaea (AOA) and bacteria (AOB) abundance and active ammonia oxidizers of activated sludge were explored under different salinities. Results showed that specific ammonium oxidation rates were significantly negative with increasing salinity. The responses of AOA and AOB populations to salt stress were distinct. AOA abundance decreased at moderate salinities (2.5, 5 and 7 g L) and increased at high salinities (10, 15, 20 and 30 g L), while AOB abundance showed opposite tendency. DNA-based stable isotope probing assays indicated AOA exclusively dominated active ammonia oxidation of test samples under different salinities. The active AOA communities retrieved were all non-halophilic and regulated by salinities. Candidatus Nitrosocosmicus exaquare and Ca. Nitrosocosmicus franklandus were the predominantly active AOA in both salt-free and salt-containing microcosms, while C-labeled Nitrososphaera viennensis and Ca. Nitrososphaera gargensis were only retrieved from the microcosms amended with 0 and 30 g L salinity, respectively.
在不同盐度下，研究了活性污泥中硝化活性、氨氧化古菌（AOA）和细菌（AOB）丰度和活性氨氧化菌的动态。结果表明，随着盐度的增加，特定氨氧化速率呈显著负相关。AOA 和 AOB 种群对盐胁迫的响应明显不同。AOA 丰度在中等盐度（2.5、5 和 7 g/L）下降低，在高盐度（10、15、20 和 30 g/L）下增加，而 AOB 丰度则呈现相反的趋势。基于 DNA 的稳定同位素探针分析表明，在不同盐度下，AOA 是测试样品中活性氨氧化的唯一优势种群。从不同盐度的试验样品中回收的活性 AOA 群落均为非嗜盐菌，受盐度调节。Candidatus Nitrosocosmicus exaquare 和 Ca. Nitrosocosmicus franklandus 是在无盐和含盐微宇宙中均占优势的主要活性 AOA，而 C 标记的 Nitrososphaera viennensis 和 Ca. Nitrososphaera gargensis 仅分别从添加 0 和 30 g/L 盐度的微宇宙中回收。
